Human Marapsin/Pancreasin Antibody

Catalog # Availability Size / Price Qty
MAB1988
MAB1988-SP
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Human Marapsin/Pancreasin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Marapsin/Pancreasin in direct ELISAs and Western blots. Reacts with an epitope within the mature chain. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Marapsin is observed.
Source
Monoclonal Mouse IgG1 Clone # 243305
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Marapsin/Pancreasin
Ala23-Lys290
Accession # Q9BQR3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Marapsin/Pancreasin (Catalog # 1988-SE)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Marapsin/Pancreasin (Catalog # 1988-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Marapsin/Pancreasin

Marapsin, pancreasin, and channel-activating protease 2 (CAP-2) are different names for the same serine protease that is expressed strongly in the pancreas (1, 2). The protein is synthesized with a signal peptide (amino acids 1‑22), a pro peptide (aa 23‑34) and a mature chain (aa 35‑290) corresponding to the serine protease domain.

References
  1. Bhagwandin, V.J. et al. (2003) J. Biol. Chem. 278:3363.
  2. Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.
Entrez Gene IDs
83886 (Human); 213171 (Mouse)
Alternate Names
CAP-2; CAPH2; channel-activating protease 2; EC 3.4.21; EC 3.4.21.-; EC 3.4.21.4; EC 3.4.21.43; Marapsin; MPN; MPNMarapsin; Pancreasin; protease, serine 27; PRSS27; serine protease 27

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