Human MALT1 Antibody

Name: Human MALT1 Antibody
Brand: R&D Systems
SKU: MAB4850
Rating: 5 (2 reviews)

Catalog #: MAB4850 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4850
MAB4850-SP

Detection of Human MALT1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MALT1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MALT1 in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # MALT1-C

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Recombinant human MALT1
Asp701-Thr808
Accession # Q9UDY8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human MALT1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MALT1 Monoclonal Antibody (Catalog # MAB4850) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MALT1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western

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Detection of Human MALT1 by Simple Western^TM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for MALT1 at approximately 98 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human MALT1 Monoclonal Antibody (Catalog # MAB4850). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MALT1

Mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT1) is an approximately 91 kDa intracellular adaptor protein that forms a complex with Bcl10 and CARMA-1. The caspase-like domain of MALT1 cleaves Bcl10 and the NF kappa B inhibitor A20. This results in NF kappa B activation following signal transduction from cell surface ITAM-containing receptors including the T cell and B cell antigen receptors. Human MALT1 shares 87% aa sequence identity with mouse and rat MALT1.

Long Name

Mucosa Associated Lymphoid Tissue Lymphoma Translocation Gene 1

Entrez Gene IDs

10892 (Human); 240354 (Mouse); 307366 (Rat)

Alternate Names

caspase-like protein; DKFZp434L132; EC 3.4.22.-; MALT associated translocation; MALT lymphoma-associated translocation; MALT1; MLT1; MLTMALT-lymphoma associated translocation; mucosa associated lymphoid tissue lymphoma translocation gene 1; mucosa-associated lymphoid tissue lymphoma translocation protein 1; Paracaspase