Human LYVE-1 Biotinylated Antibody

Catalog #: BAF2089
2 Citations Datasheet / COA / SDS

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BAF2089

All LYVE-1 Antibodies

Product Details

Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LYVE-1 Biotinylated Antibody Summary

Specificity

Detects LYVE‑1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse LYVE-1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LYVE-1
Ser24-Thr238
Accession # Q9Y5Y7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Purity

Antigen Affinity-purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human LYVE‑1 (Catalog # 2089-LY)

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human tonsil

Human LYVE-1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human LYVE‑1 Antibody (Catalog # MAB20891)

Standard: Recombinant Human LYVE-1 Protein, CF (Catalog # 2089-LY)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LYVE-1

Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a receptor of HA, a linear high molecular weight polymer composed of alternating units of D‑glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1-3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity.

LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9-11).

References

Knudson, C.B. and W. Knudson (1993) FASEB J. 7:1233.
Evered, D and J. Whelan (1989) Ciba Found. Symp. 143:1.
Laurent, T.C. and J.R.F. Fraser (1992) FASEB J. 6:2397.
Banerji, S. et al. (1999) J. Cell Biol. 144:789.
Prevo, R. et al. (2001) J. Biol. Chem. 276:19420.
Jackson, D.J. et al. (2001)Trends Immunol. 22:317.
Zhou, B. et al. (2000) J. Biol. Chem. 275:37733.
Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
Breiteneder-Gellef, S. et al. (1999) Am. J. Pathol. 154:385.
Wiggle, J.T. and G. Oliver (1999) Cell 98:769.

Long Name

Lymphatic Vessel Endothelial Hyaluronan Receptor 1

Entrez Gene IDs

10894 (Human); 114332 (Mouse); 293186 (Rat)

Alternate Names

cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP1; CRSBP-1; extracellular link domain containing 1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; HAR; Hyaluronic acid receptor; lymphatic vessel endothelial hyaluronan receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1; LYVE-1; UNQ230/PRO263; XLKD1

Product Datasheets

Product Datasheet

COA

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Citations for Human LYVE-1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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The cortical actin network regulates avidity-dependent binding of hyaluronan by the Lymphatic Vessel Endothelial receptor LYVE-1
Authors: TA Stanly, M Fritzsche, S Banerji, D Shrestha, F Schneider, C Eggeling, DG Jackson
J. Biol. Chem., 2020-02-07;0(0):.
Species: Human
Sample Types: Cell Culture Lysates
Applications: Immunoprecipitation
Inhibition of the mammalian target of rapamycin impedes lymphangiogenesis.
Authors: Huber S, Bruns CJ, Schmid G, Hermann PC, Conrad C, Niess H, Huss R, Graeb C, Jauch KW, Heeschen C, Guba M
Kidney Int., 2007-02-14;71(8):771-7.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry