Human LYVE-1 Antibody Summary
Ser24-Thr238
Accession # Q9Y5Y7
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![LYVE‑1 antibody in Human Tonsil by Immunohistochemistry (IHC-P). LYVE‑1 antibody in Human Tonsil by Immunohistochemistry (IHC-P).](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/LYVE-1_AF2089_Immunohistochemistry_6732.jpg)
LYVE‑1 in Human Tonsil. LYVE‑1 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Goat Anti-Human LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2089) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
![Detection of Human LYVE‑1 antibody by Western Blot. Detection of Human LYVE‑1 antibody by Western Blot.](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/LYVE-1_AF2089_Western_Blot_5169.jpg)
Detection of Human LYVE‑1 by Western Blot. Western blot shows lysates of human liver and spleen tissue. PVDF membrane was probed with 0.25-1 µg/mL of Goat Anti-Human LYVE-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2089) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for LYVE-1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LYVE-1
Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a receptor of HA, a linear high molecular weight polymer composed of alternating units of D‑glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1-3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity.
LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9-11).
- Knudson, C.B. and W. Knudson (1993) FASEB J. 7:1233.
- Evered, D and J. Whelan (1989) Ciba Found. Symp. 143:1.
- Laurent, T.C. and J.R.F. Fraser (1992) FASEB J. 6:2397.
- Banerji, S. et al. (1999) J. Cell Biol. 144:789.
- Prevo, R. et al. (2001) J. Biol. Chem. 276:19420.
- Jackson, D.J. et al. (2001)Trends Immunol. 22:317.
- Zhou, B. et al. (2000) J. Biol. Chem. 275:37733.
- Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
- Breiteneder-Gellef, S. et al. (1999) Am. J. Pathol. 154:385.
- Wiggle, J.T. and G. Oliver (1999) Cell 98:769.
Product Datasheets
Citations for Human LYVE-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Piezo1 regulates meningeal lymphatic vessel drainage and alleviates excessive CSF accumulation
Authors: Choi, D;Park, E;Choi, J;Lu, R;Yu, JS;Kim, C;Zhao, L;Yu, J;Nakashima, B;Lee, S;Singhal, D;Scallan, JP;Zhou, B;Koh, CJ;Lee, E;Hong, YK;
Nature neuroscience
Species: Human hepegivirus
Sample Types: Cell Lysates
Applications: Western Blot -
Venous-plexus-associated lymphoid hubs support meningeal humoral immunity
Authors: Fitzpatrick, Z;Ghabdan Zanluqui, N;Rosenblum, JS;Tuong, ZK;Lee, CYC;Chandrashekhar, V;Negro-Demontel, ML;Stewart, AP;Posner, DA;Buckley, M;Allinson, KSJ;Mastorakos, P;Chittiboina, P;Maric, D;Donahue, D;Helmy, A;Tajsic, T;Ferdinand, JR;Portet, A;Peñalver, A;Gillman, E;Zhuang, Z;Clatworthy, MR;McGavern, DB;
Nature
Species: Human hepegivirus
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Single-cell atlas of the human neonatal small intestine affected by necrotizing enterocolitis
Authors: Egozi, A;Olaloye, O;Werner, L;Silva, T;McCourt, B;Pierce, RW;An, X;Wang, F;Chen, K;Pober, JS;Shouval, D;Itzkovitz, S;Konnikova, L;
PLoS biology
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Lymphatics act as a signaling hub to regulate intestinal stem cell activity
Authors: RE Niec, T Chu, M Schernthan, S Gur-Cohen, L Hidalgo, HA Pasolli, KA Luckett, Z Wang, SR Bhalla, F Cambuli, RP Kataru, K Ganesh, BJ Mehrara, D Pe'er, E Fuchs
Cell Stem Cell, 2022-06-20;29(7):1067-1082.e18.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Lipid droplet degradation by autophagy connects mitochondria metabolism to Prox1-driven expression of lymphatic genes and lymphangiogenesis
Authors: O Meçe, D Houbaert, ML Sassano, T Durré, H Maes, M Schaaf, S More, M Ganne, M García-Cab, M Borri, J Verhoeven, M Agrawal, K Jacobs, G Bergers, S Blacher, B Ghesquière, M Dewerchin, JV Swinnen, S Vinckier, MS Soengas, P Carmeliet, A Noël, P Agostinis
Nature Communications, 2022-05-19;13(1):2760.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Pilot Study of Anti-Th2 Immunotherapy for the Treatment of Breast Cancer-Related Upper Extremity Lymphedema
Authors: BJ Mehrara, HJ Park, RP Kataru, J Bromberg, M Coriddi, JE Baik, J Shin, C Li, MR Cavalli, EM Encarnacio, M Lee, KJ Van Zee, E Riedel, JH Dayan
Biology, 2021-09-18;10(9):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
A novel suprachoroidal microinvasive glaucoma implant: in vivo biocompatibility and biointegration
Authors: I Grierson, D Minckler, MK Rippy, AJ Marshall, N Collignon, J Bianco, B Detry, MA Johnstone
BMC Biomed Eng, 2020-10-14;2(0):10.
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHC -
TGF-beta and TNF-alpha cooperatively induce mesenchymal transition of lymphatic endothelial cells via activation of Activin signals
Authors: Y Yoshimatsu, S Kimuro, J Pauty, K Takagaki, S Nomiyama, A Inagawa, K Maeda, KA Podyma-Ino, K Kajiya, YT Matsunaga, T Watabe
PLoS ONE, 2020-05-01;15(5):e0232356.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
The cortical actin network regulates avidity-dependent binding of hyaluronan by the Lymphatic Vessel Endothelial receptor LYVE-1
Authors: TA Stanly, M Fritzsche, S Banerji, D Shrestha, F Schneider, C Eggeling, DG Jackson
J. Biol. Chem., 2020-02-07;0(0):.
Species: Human
Sample Types: Cell Culture Lysates
Applications: Immunoprecipitation -
Receptor-Interacting Protein Kinase 1 Promotes Cholangiocarcinoma Proliferation And Lymphangiogenesis Through The Activation Protein 1 Pathway
Authors: CZ Li, YX Lin, TC Huang, JY Pan, GX Wang
Onco Targets Ther, 2019-11-01;12(0):9029-9040.
Species: Xenograft
Sample Types: Whole Tissue
Applications: IHC-P -
Epithelial Membrane Protein-2 (EMP2) Antibody Blockade Reduces Corneal Neovascularization in an In Vivo Model
Authors: MM Sun, AM Chan, SM Law, S Duarte, D Diaz-Aguil, M Wadehra, LK Gordon
Invest. Ophthalmol. Vis. Sci., 2019-01-02;60(1):245-254.
Species: Human
Sample Types: Tissue Homogenates
Applications: IHC -
Lymph nodes are sites of prolonged bacterial persistence during Mycobacterium tuberculosis infection in macaques
Authors: SKC Ganchua, AM Cadena, P Maiello, HP Gideon, AJ Myers, BF Junecko, EC Klein, PL Lin, JT Mattila, JL Flynn
PLoS Pathog., 2018-11-01;14(11):e1007337.
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Tissue
Applications: IHC -
Induced dural lymphangiogenesis facilities soluble amyloid-beta clearance from brain in a transgenic mouse model of Alzheimer's disease
Authors: YR Wen, JH Yang, X Wang, ZB Yao
Neural Regen Res, 2018-04-01;13(4):709-716.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Single-Cell RNA-Seq Reveals the Transcriptional Landscape and Heterogeneity of Aortic Macrophages in Murine Atherosclerosis
Authors: C Cochain, E Vafadarnej, P Arampatzi, P Jaroslav, H Winkels, K Ley, D Wolf, AE Saliba, A Zernecke
Circ. Res., 2018-03-15;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Silencing of Discoidin Domain Receptor-1 (DDR1) Concurrently Inhibits Multiple Steps of Metastasis Cascade in Gastric Cancer
Authors: R Yuge, Y Kitadai, H Takigawa, T Naito, N Oue, W Yasui, S Tanaka, K Chayama
Transl Oncol, 2018-03-13;11(3):575-584.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
The effect of neoadjuvant chemotherapy on the correlation of tumor-associated macrophages with CD31 and LYVE-1
Authors: I Mitrofanov, M Zavyalova, V Riabov, N Cherdyntse, J Kzhyshkows
Immunobiology, 2017-10-31;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
VIPAR, a quantitative approach to 3D histopathology applied to lymphatic malformations
Authors: R Hägerling, D Drees, A Scherzinge, C Dierkes, S Martin-Alm, S Butz, K Gordon, M Schäfers, K Hinrichs, P Ostergaard, D Vestweber, T Goerge, S Mansour, X Jiang, PS Mortimer, F Kiefer
JCI Insight, 2017-08-17;2(16):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4
Authors: LD Volk-Drape, KL Hall, AC Wilber, S Ran
PLoS ONE, 2017-06-09;12(6):e0179257.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
TGF-?1 Is Present at High Levels in Wound Fluid from Breast Cancer Patients Immediately Post-Surgery, and Is Not Increased by Intraoperative Radiation Therapy (IORT)
PLoS ONE, 2016-09-02;11(9):e0162221.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
Authors: Riordan D, Varma S, West R, Brown P
PLoS ONE, 2015-07-15;10(7):e0128975.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Adrenomedullin blockade suppresses growth of human hormone-independent prostate tumor xenograft in mice.
Authors: Berenguer-Daize C, Boudouresque F, Bastide C, Tounsi A, Benyahia Z, Acunzo J, Dussault N, Delfino C, Baeza N, Daniel L, Cayol M, Rossi D, El Battari A, Bertin D, Mabrouk K, Martin P, Ouafik L
Clin Cancer Res, 2013-10-07;19(22):6138-50.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
An in vivo platform for tumor biomarker assessment.
Authors: Servais EL, Suzuki K, Colovos C
PLoS ONE, 2011-10-26;6(10):e26722.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, IHC -
Notch alters VEGF responsiveness in human and murine endothelial cells by direct regulation of VEGFR-3 expression.
Authors: Shawber CJ, Funahashi Y, Francisco E, Vorontchikhina M, Kitamura Y, Stowell SA, Borisenko V, Feirt N, Podgrabinska S, Shiraishi K, Chawengsaksophak K, Rossant J, Accili D, Skobe M, Kitajewski J
J. Clin. Invest., 2007-11-01;117(11):3369-82.
Species: Human, Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Effects of acute exercise, exercise training, and diabetes on the expression of lymphangiogenic growth factors and lymphatic vessels in skeletal muscle.
Authors: Kivela R, Silvennoinen M, Lehti M, Kainulainen H, Vihko V
Am. J. Physiol. Heart Circ. Physiol., 2007-08-31;293(4):H2573-9.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium.
Authors: Silverman MD, Haas CS, Rad AM, Arbab AS, Koch AE
Arthritis Rheum., 2007-06-01;56(6):1817-26.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
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