Human LYPD8 Antibody

Catalog #: MAB9087 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9087-100
MAB9087-SP

Detection of LYPD8 in HEK293 Human Cell Line Transfected with Human LYPD8 and eGFP by Flow Cytometry.

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Human LYPD8 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LYPD8 in direct ELISAs. Stains human LYPD8 transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG₁ Clone # 961703

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell line HEK293-derived transfected with human LYPD8
Met1-Asn215
Accession # Q6UX82

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of LYPD8 in HEK293 Human Cell Line Transfected with Human LYPD8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human LYPD8 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human LYPD8 Monoclonal Antibody (Catalog # MAB9087) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry

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LYPD8 in HEK293 Human Cell Line. LYPD8 was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human LYPD8 Monoclonal Antibody (Catalog # MAB9087) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LYPD8

Ly6/PLAUR domain containing 8 (LYPD8), is a GPI-linked protein with structural similarity to the urokinase-type plasminogen activator receptor (uPAR) (1). Mature human LYPD8 contains one uPAR/Ly6 domain and a Ser/Thr/Pro-rich (STP) region that may serve as a target for protease mediated shedding as has been shown for the related C4.4A/LYPD3 molecule (2, 3). Mature human LYPD8 shares 40% amino acid sequence identity with mouse and rat LYPD8.

References