Human LAP (TGF-beta 1) Antibody

Catalog #: MAB2461
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2461-SP
MAB2461-500
MAB2461-100

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LAP (TGF-beta 1) Antibody Summary

Species Reactivity

Human

Specificity

Detects human LAP (TGF-beta 1) and Latent TGF-beta 1 in ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 9005

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Latent TGF‑ beta 1
Leu30-Ser390
Accession # P01137

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Human LAP TGF-beta 1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human LAP TGF-beta 1 Biotinylated Antibody (Catalog # BAM2462)

Standard: Recombinant Human LAP (TGF-beta 1) Protein (Catalog # 246-LP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAP (TGF-beta 1)

The TGF-beta family includes several related proteins (70 - 80% sequence homology) from mammalian, avian or Xenopus systems that are now designated TGF-beta 1, TGF-beta 2, TGF-beta 1.2, TGF-beta 3, TGF-beta 4, and TGF-beta 5. These proteins are secreted by cells in the form of an inactive complex, referred to as Latent TGF-beta, that consists of TGF-beta associated non-covalently with a Latency-associated peptide (LAP). These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF-beta family members are naturally associated with their own distinct LAPs.

Long Name

Latency-associated Peptide

Entrez Gene IDs

7040 (Human)

Alternate Names

CED; DPD1; LAP (TGFbeta 1); LAP (TGF-beta 1); LAP; TGFB; TGFB1; TGFbeta; transforming growth factor beta 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human LAP (TGF-beta 1) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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BMPR2 acts as aï¿½gatekeeper to protect endothelial cells from increased TGF&betaï¿½responses and altered cell mechanics
Authors: C Hiepen, J Jatzlau, S Hildebrand, B Kampfrath, M Goktas, A Murgai, JL Cuellar Ca, R Haag, C Ruppert, G Sengle, EA Cavalcanti, KG Blank, P Knaus
PLoS Biol., 2019-12-11;17(12):e3000557.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
P311 binds to the latency associated protein and downregulates the expression of TGF-beta1 and TGF-beta2.
Authors: Paliwal S, Shi J, Dhru U, Zhou Y, Schuger L
Biochem. Biophys. Res. Commun., 2004-03-19;315(4):1104-9.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot