Human L1CAM Antibody

Detection of Human L1CAM by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human brain (motor cortex). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human L1CAM Monoclonal Antibody (Catalog # MAB777) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for L1CAM at approximately 240 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human L1CAM by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for L1CAM at approximately 337 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human L1CAM Monoclonal Antibody (Catalog # MAB777). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Detection of Mouse L1CAM by Immunohistochemistry Analysis of L1 and pCK2 alpha immunofluorescence intensities rostral, central and caudal to the lesion site of the injured spinal cord at 6 weeks post-SCI in mice treated with trimebutine or honokiol. (A,C) Representative images of L1 (A) and pCK2 alpha (C) rostral (1 mm to the lesion center), central and caudal (1 mm to the lesion center) to the lesion site. (B,D) Quantitative analysis of L1 (B) and pCK2 alpha (D) immunoreactivities. *P<0.05, **P<0.01 versus the vehicle control; one-way ANOVA with Tukey's post hoc test; n=3 mice/group. HNK, honokiol; TMB, trimebutine; VC, vehicle control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28714852), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse L1CAM by Western Blot Activation of L1-mediated intracellular signaling cascades in cultured mouse cerebellar granule cells treated with trimebutine. (A-E) Protein levels of L1 (A), phosphorylation levels of Akt1 (B) and Erk1/2 (C), protein levels of mTOR (D), and the ratio of Bcl-2/Bax levels (E) in cultured mouse cerebellar granule cells treated with trimebutine (TMB) at concentrations of 0, 5, 10 and 20 nM. *P<0.05, **P<0.01, ***P<0.001 versus the vehicle control; one-way ANOVA with Tukey's post hoc test; three independent experiments. Blots in C-E are from the same gel and therefore have the same beta -actin loading controls. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28714852), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse L1CAM by Western Blot Activation of L1-mediated intracellular signaling cascades at 6 weeks after SCI in mice treated with trimebutine or honokiol. Western blot analysis of L1 (A), phosphorylation levels of Akt1 (B), Erk1/2 (C), CK2 alpha (D) and mTOR (E), protein levels of PTEN (F), p53 (G) and GFAP (H), and Bcl-2/Bax ratio (I) of mice treated with vehicle control (VC), trimebutine (TMB) or honokiol (HNK) at 5 mm rostral to the lesion center, the lesion center, and 5 mm caudal to the lesion center. *P<0.05, **P<0.01 versus the vehicle control; one-way ANOVA with Tukey's post hoc test; n=4 mice/group. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28714852), licensed under a CC-BY license. Not internally tested by R&D Systems.