Human Ku80/XRCC5 Antibody

Catalog #: AF5619 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5619
AF5619-SP

Detection of Human Ku80/XRCC5 by Western Blot.

2 Images

All Ku80/XRCC5 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Ku80/XRCC5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Ku80 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Ku80
Asn402-Lys565
Accession # P13010

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

1-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human Ku80/XRCC5 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, A549 human lung carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Ku80/XRCC5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5619) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Ku80/XRCC5 at approximately 86 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

View Larger

Ku80/XRCC5 in Human Breast Cancer Tissue. Ku80/XRCC5 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human Ku80/XRCC5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5619) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ku80/XRCC5

Ku80 (Lupus Ku autoantigen protein p86; also ATP-dependent DNA helicase 2 subunit 2 and Ku86) is a ubiquitous 80 - 85 kDa nuclear protein that serves as a component of DNA-dependent protein kinase. In particular, it heterodimerizes with Ku70 to form a complex that binds DNA ends and select sequences, and which recruits DNA-PKcs for the purpose of phosphorylating multiple chromatin-bound proteins, including p53, Oct-1 and Sp-1. The Ku:DNA-PKcs complex participates in DNA repair, DNA replication, and VDJ recombination. Human Ku80 is 732 amino acids (aa) in length. It contains a Ku domain (aa 244 - 543) with a DNA binding motif (aa 257 - 268), and a C-terminal DNA-PKcs-binding sequence (aa 720 - 728). Over aa 402 - 565, Ku80 shares 77% aa identity with mouse Ku80.

Long Name

Lupus Ku Autoantigen Protein 86

Entrez Gene IDs

7520 (Human); 22596 (Mouse); 363247 (Rat)

Alternate Names

80kDa; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC85; CTCBF; EC 3.6.4.-; FLJ39089; G22P2; KARP1; KARP-1; Ku80; Ku86 autoantigen related protein 1,86 kDa subunit of Ku antigen; Ku86; Ku86DNA repair protein XRCC5; KUB2; NFIV; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining)CTC box-binding factor 85 kDa subunit; X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining; Ku autoantigen, 80kD); X-ray repair cross-complementing protein 5; X-ray repair, complementing defective, repair in Chinese hamster; XRCC5