Human Kallikrein 8/Neuropsin Antibody

Catalog #: MAB1719
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1719
MAB1719-SP

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Kallikrein 8/Neuropsin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Kallikrein 8/Neuropsin in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Kallikrein 3, 5, 11, or rhHGFA is observed.

Source

Monoclonal Mouse IgG_2B Clone # 189004

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 8/Neuropsin
Gln29-Gly260 (predicted)
Accession # O60259

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Kallikrein 8/Neuropsin (Catalog # 2025-SE)

Immunohistochemistry

5-25 µg/mL

See below

Immunocytochemistry

1-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Kallikrein 8/Neuropsin in Human Brain. Kallikrein 8/Neuropsin was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human Kallikrein 8/Neuropsin Monoclonal Antibody (Catalog # MAB1719) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry

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Kallikrein 8/Neuropsin in SH-SY5Y Human Cell Line. Kallikrein 8/Neuropsin was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human Kallikrein 8/Neuropsin Monoclonal Antibody (Catalog # MAB1719) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 8/Neuropsin

Kallikrein 8 (KLK8), also known as neuropsin or ovasin, is a member of the human tissue kallikrein family (1). Two alternatively spliced forms exist, resulting in 260 (isoform 1) and 305 (isoform 2) amino acid (aa) sequences, respectively (2). Isoform 1 consists of a signal peptide (residues 1 to 28), a short pro peptide
(aa 29‑32) and the mature chain (aa 33-260). Isoform 2 is identical to isoform 1, except that a 45 aa segment is inserted in isoform 2 between aa 23 and 24 in isoform 1. Isoform 1 is predominantly expressed in pancreas whereas isoform 2 is preferentially expressed in adult brain and hippocampus, although both forms are expressed in fetal brain and placenta in comparable levels. The brain function of KLK8 seems evident in neuropsin knockout mice that showed abnormalities of synapses and neurons and predisposation to global seizure activity (3, 4). KLK8 is a novel marker for ovarian and cervical cancer carcinomas (5, 6). Recombinant human KLK8, after being activated by lysyl endopeptidase, can cleave fibronectin and several small peptide substrates (7, 8).

References

Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
Mitsui, S. et al. (1999) Eur. J. Biochem. 260:627.
Hirata, A. et al. (2001) Mol. Cell. Neurosci. 17:600.
Davies, B. et al. (2001) J. Neurosci. 21:6993.
Kishi, T. et al. (2003) Cancer Res. 63:2771.
Cane, S. et al. (2004) Am. J. Obstet. Gynecol. 190:60.
Oka, T. et al. (2002) J. Biol. Chem. 277:14724.
Shimizu, C. et al. (1998) J. Biol. Chem. 273:11189.

Entrez Gene IDs

11202 (Human)

Alternate Names

EC 3.4.21; EC 3.4.21.118; hK8; HNP; kallikrein 8 (neuropsin/ovasin); Kallikrein 8; kallikrein-related peptidase 8; KLK8 protein type 1; KLK8 protein type 2; KLK8; neuropsin type 1; neuropsin type 2; Neuropsin; NP; NRPN; Ovasin; PRSS19; PRSS19kallikrein-8; Serine protease 19; Serine protease TADG-14; TADG14neuropsin; Tumor-associated differentially expressed gene 14 protein

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Product Datasheet

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Citation for Human Kallikrein 8/Neuropsin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Classification of node-positive melanomas into prognostic subgroups using keratin, immune, and melanogenesis expression patterns
Authors: D Netanely, S Leibou, R Parikh, N Stern, H Vaknine, R Brenner, S Amar, RH Factor, T Perluk, J Frand, E Nizri, D Hershkovit, V Zemser-Wer, C Levy, R Shamir
Oncogene, 2021-02-09;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC