Human Kallikrein 2 Antibody

Catalog #: MAB4104
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4104
MAB4104-SP

All Kallikrein 2 Antibodies

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Kallikrein 2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Kallikrein 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Kallikrein 1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, or B1 is observed.

Source

Monoclonal Mouse IgG1 Clone # 426723

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Kallikrein 2 (Catalog # 4104-SE).

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Kallikrein 2 (Catalog # 4104-SE), see our available Western blot detection antibodies

Neutralization

Measured by its ability to neutralize Recombinant Human Kallikrein 2 (2 µg/mL, Catalog # 4104-SE) cleavage of the fluorogenic peptide substrate PFR-AMC (0.1 mM). The Neutralization Dose (ND₅₀) is typically 3.4 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Kallikrein 2

Kallikrein 2 (KLK2) is a secreted serine protease that is highly expressed in the human prostate gland (1). It is also known as the prostate-specific glandular kallikrein. The enzyme is highly specific for cleavage after arginine residues. KLK2 is able to activate the urokinase-type plasminogen activator (2). KLK2 is inhibited by serpins such as protein C inhibitor, antichymotrypsin, and plasminogen activator inhibitor 1 (3‑5). KLK2 is structurally related to KLK3, the prostate-specific antigen (PSA). Like PSA, KLK2 is considered to be a biomarker for prostate cancer (6).

References

Chapelaine, P. et al. (1988) FEBS Lett. 236:205.
Frenette, G. et al. (1997) Int. J. Cancer 71:897.
Deperthes, D. et al. (1995) Biochim. Biophys. Acta 1245:311.
Grauer, L.S. et al. (1998) J. Androl. 19:407.
Mikolajczyk S.D. et al. (1999) Cancer Res. 59:3927.
Sardana, G. et al. (2008) Clin. Chem. 54:1951.

Entrez Gene IDs

3817 (Human); 24841 (Rat); 102139474 (Cynomolgus Monkey)

Alternate Names

EC 3.4.21; EC 3.4.21.35; glandular kallikrein 2; Glandular kallikrein-1; hGK-1; hK2; Kallikrein 2; kallikrein 2, prostatic; kallikrein-2; kallikrein-related peptidase 2; Klk1c2; KLK2; KLK2A2; MGC12201; Tissue kallikrein-2; Ton

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Kallikrein 2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Direct B cell stimulation by dendritic cells in a mouse model of lupus.
Authors: Wan S, Zhou Z, Duan B, Morel L
Arthritis Rheum., 2008-06-01;8(0):1741-50.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization