Human Kallikrein 15 Antibody

Catalog #: AF2540 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2540
AF2540-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Kallikrein 15 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Kallikrein 15 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) Kallikrein 2, rhKallikrein 8, and rhKLK11 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 15 isoform 1
Asp17-Asn255
Accession # AAI44047

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Kallikrein 15

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human ovarian cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Kallikrein 15, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 15

Kallikrein 15, encoded by the KLK15 gene, is a member of the tissue Kallikrein family of Serine proteases. It is a secreted protein found in prostate, colon, thyroid tissues, breast milk and seminal plasma. Whether it has a trypsin-like activity or not remains controversial. Several splice forms exist. The 250 aa rhKLK15 has a 10‑His tag at the C-terminus. It predicts a molecular mass of 28 kDa and migrates as a 39 kDa band in SDS-PAGE under reducing conditions. The amino acid sequence of human KLK15 is 99%, 77% and 77% identical to that of chimpanzee, canine and mouse, respectively.

Entrez Gene IDs

55554 (Human)

Alternate Names

ACO protease; ACO; EC 3.4.21; EC 3.4.21.-; EC 3.4.21.4; HSRNASPH; Kallikrein 15; kallikrein-15; kallikrein-like serine protease; kallikrein-related peptidase 15; KLK15; Prostinogen