Human Insulin Antibody

Catalog # Availability Size / Price Qty
MAB8056
MAB8056-SP
Insulin in Human Pancreas.
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Human Insulin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Insulin in ELISA.
Source
Monoclonal Mouse IgG1 Clone # 873724
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
The construct consists of the alpha mating factor signal followed by a 10 aa peptide leader, B chain aa 1-29, AAK spacer A chain aa 1-21.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Insulin antibody in Human Pancreas by Immunohistochemistry (IHC-P). View Larger

Insulin in Human Pancreas. Insulin was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Insulin Monoclonal Antibody (Catalog # MAB8056) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Insulin

Insulin is a disulfide-linked heterodimeric protein secreted by the pancreatic Islets of Langerhans. Mature insulin is generated by the proteolytic removal of a peptide from proinsulin. It is involved in the regulation of glucose metabolism through interactions with the Insulin Receptor.

Entrez Gene IDs
3630 (Human); 16333 (Mouse); 397415 (Porcine); 280829 (Bovine); 483665 (Canine)
Alternate Names
IDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin

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Human Insulin Antibody
By Anonymous on 10/17/2018
Application: ELISA Sample Tested: Cancer Tissue Species: Human