Human IL-9R Antibody

Name: Human IL-9R Antibody
Brand: R&D Systems
SKU: MAB290
Rating: 5 (2 reviews)

Catalog #: MAB290
4 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB290
MAB290-SP

Detection of IL‑9 R in Human Monocytes by Flow Cytometry.

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All IL-9R Antibodies

Product Details

Citations (4)

FAQs

Supplemental Products

Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-9R Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-9 R in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-4 R, rhIL-5 R alpha, rhIL-5 R beta, rhIL‑13 R alpha 1, rhIL-13 R alpha 2, or recombinant mouse IL-9 R is observed.

Source

Monoclonal Mouse IgG₁ Clone # 33423

Purification

Protein A or G purified from ascites

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑9 R

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Neutralization

Measured by its ability to neutralize IL‑9-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND₅₀) is typically 2-4 µg/mL in the presence of 2 ng/mL Recombinant Human IL‑9.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IL‑9 R in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Mouse Anti-Human IL-9 R Subunit Monoclonal Antibody (Catalog # MAB290, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

Neutralization

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Cell Proliferation Induced by IL‑9 and Neutralization by Human IL‑9 R Antibody. Recombinant Human IL-9 (Catalog # 209-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-9 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-9 R Subunit Mono-clonal Antibody (Catalog # MAB290). The ND₅₀ is typically 2-4 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-9R

Interleukin 9 Receptor (IL-9 R) belongs to the hematopoietin receptor superfamily and is the binding subunit of the heterodimeric IL-9 receptor complex. The other subunit is the common gamma chain shared with the receptors for IL-2, IL-4, IL-7, and IL-15. IL-9 R is expressed by T cells, neutrophils, mast cells, and macrophages.

Long Name

Interleukin 9 Receptor

Entrez Gene IDs

3581 (Human); 16199 (Mouse)

Alternate Names

CD129 antigen; CD129; IL-9 R; IL-9 receptor; IL9R; IL-9R; interleukin 9 receptor; interleukin-9 receptor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human IL-9R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Interleukin-9 regulates macrophage activation in the progressive multiple sclerosis brain
Authors: G Donninelli, I Saraf-Sini, V Mazziotti, A Capone, MG Grasso, L Battistini, R Reynolds, R Magliozzi, E Volpe
J Neuroinflammation, 2020-05-06;17(1):149.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
IL-9 promotes the pathogenesis of ulcerative colitis through STAT3/SOCS3 signaling
Authors: L Tian, Y Li, J Zhang, R Chang, J Li, L Huo
Biosci. Rep., 2018-11-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Expression of IL-9 receptor alpha chain on human germinal center B cells modulates IgE secretion.
Authors: Fawaz LM, Sharif-Askari E, Hajoui O, Soussi-Gounni A, Hamid Q, Mazer BD
J. Allergy Clin. Immunol., 2007-10-24;120(5):1208-15.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Interleukin-9 induces goblet cell hyperplasia during repair of human airway epithelia.
Authors: Vermeer PD, Harson R, Einwalter LA, Moninger T
Am. J. Respir. Cell Mol. Biol., 2003-03-01;28(3):286-95.
Species: Human
Sample Types: Whole Cells
Applications: ICC