Home / IL-36 gamma/IL-1F9 / Human IL-36 gamma /IL-1F9 Biotinylated Antibody

Human IL-36 gamma /IL-1F9 Biotinylated Antibody

Catalog #: BAF2320
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BAF2320
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-36 gamma /IL-1F9 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑36 gamma /IL‑1F9 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) IL-36 alpha is observed, and less than 1% cross-reactivity with rhIL-1 alpha, rhIL-1 beta, rhIL-18, rhIL-36Ra, rhIL-37, and rhIL-36 beta is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL‑36 gamma /IL‑1F9
Met1-Asp169
Accession # Q9NZH8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑36 gamma /IL‑1F9 (Catalog # 2320-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36 gamma/IL-1F9

Human interleukin 1 family member #9 [IL-1F9; also named IL-1 epsilon  (epsilon) and IL-1H1] is a member of the IL-1 family, which includes IL-1 beta, IL-1 alpha, IL-1ra, IL-18 and IL-1F5 through F10 (1‑4). All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-1F9 is synthesized as a 19 kDa, 169 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site (1, 2, 5). The molecule is secreted when transfected into 293-T cells (5). Human to mouse, IL-1F9 has 53% aa sequence identity. Within the family, IL-1F9 shares 30%, 23%, 33%, 57%, 35%, 45%, and 32% aa sequence identity with IL-1 ra, IL-1 beta, IL-1F5, F6, F7, F8, and F10, respectively. Cells reported to express IL-1F9 include Langerhans cells, keratinocytes/stratified squamous epithelium, plus Chief cells and Parietal cells of the stomach (4, 5). The receptor for IL‑1F9 is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (6). Recombinant IL-1F9, along with IL-1F8 and IL-1F6, has been shown to activate the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner.

References
  1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  3. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
  4. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  5. Debets, R. et al. (2001) J. Immunol. 167:1440.
  6. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
Long Name
Interleukin 36 gamma/Interleukin 1 Family 9
Entrez Gene IDs
56300 (Human); 215257 (Mouse); 499744 (Rat)
Alternate Names
IL-1 epsilon; IL-1 H1; IL-1 Related Protein 2; IL-1(EPSILON); IL1E; IL-1-epsilon; IL1F9; IL-1F9; IL1H1; IL-1H1; IL-1-Related Protein 2; IL1RP2; IL-1rp2; IL36 gamma; IL-36 gamma; IL36G; interleukin 1 family, member 9; interleukin 1-related protein 2; Interleukin 36, Gamma; Interleukin-1 epsilon; interleukin-1 family member 9; Interleukin-1 homolog 1; Interleukin-36 Gamma

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human IL-36 gamma /IL-1F9 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

  1. The Proinflammatory Cytokine IL-36 gamma Is a Global Discriminator of Harmless Microbes and Invasive Pathogens within Epithelial Tissues
    Authors: Thomas Macleod, Joseph S. Ainscough, Christina Hesse, Sebastian Konzok, Armin Braun, Anna-Lena Buhl et al.
    Cell Reports
  2. Cathepsin S is the major activator of the psoriasis-associated proinflammatory cytokine IL-36?
    Authors: JS Ainscough, T Macleod, D McGonagle, R Brakefield, JM Baron, A Alase, M Wittmann, M Stacey
    Proc. Natl. Acad. Sci. U.S.A, 2017-03-13;114(13):E2748-E2757.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Alarmin Function of Cathelicidin Antimicrobial Peptide LL37 through IL-36 gamma Induction in Human Epidermal Keratinocytes
    Authors: Na Li, Kenshi Yamasaki, Rumiko Saito, Sawako Fukushi-Takahashi, Ryoko Shimada-Omori, Masayuki Asano et al.
    The Journal of Immunology
  4. Inter-regulation of Th17 cytokines and the IL-36 cytokines in vitro and in vivo: implications in psoriasis pathogenesis.
    Authors: Carrier Y, Ma HL, Ramon HE, Napierata L, Small C, O'Toole M, Young DA, Fouser LA, Nickerson-Nutter C, Collins M, Dunussi-Joannopoulos K, Medley QG
    J. Invest. Dermatol., 2011-09-01;131(12):2428-37.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. IL-1F5, -F6, -F8, and -F9: A Novel IL-1 Family Signaling System That Is Active in Psoriasis and Promotes Keratinocyte Antimicrobial Peptide Expression.
    Authors: Johnston A, Xing X, Guzman AM, Riblett M, Loyd CM, Ward NL, Wohn C, Prens EP, Wang F, Maier LE, Kang S, Voorhees JJ, Elder JT, Gudjonsson JE
    J. Immunol., 2011-01-17;186(4):2613-22.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  6. Cytokine induced 3-D organotypic psoriasis skin model demonstrates distinct roles for NF-kappa B and JAK pathways in disease pathophysiology
    Authors: Todorovic, McDonald HA, Hoover P et al.
    Experimental dermatology

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human IL-36 gamma /IL-1F9 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Human IL-36 gamma /IL-1F9 Biotinylated Antibody and earn rewards!

Have you used Human IL-36 gamma /IL-1F9 Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review