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Human IL-1 beta /IL-1F2 Antibody

Catalog #: AB-201-NA
6 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AB-201-NA
Detection of Human and Mouse IL‑1 beta /IL‑1F2 by Western Blot.
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Citations (6)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-1 beta /IL-1F2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑1 beta /IL‑1F2 in ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant rhesus monkey is observed, and approximately 50% cross-reactivity with recombinant mouse IL‑1 beta, recombinant rat IL‑1 beta, recombinant canine IL‑1 beta, recombinant equine IL‑1 beta, recombinant feline IL‑1 beta, and approximately 20% cross-reactivity with recombinant porcine IL-1 beta and recombinant cotton rat IL-1 beta is observed.
Source
Polyclonal Goat IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 0.05-0.1 µg/mL in the presence of 50 pg/mL Recombinant Human IL‑1 beta /IL‑1F2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse IL-1 beta /IL-1F2 antibody by Western Blot. View Larger

Detection of Human and Mouse IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with PMA and LPS and RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-1 beta /IL-1F2 Polyclonal Antibody (Catalog # AB-201-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-1 beta /IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry IL-1 beta /IL-1F2 antibody in Human Peripheral Blood Mononuclear Cells by Immunocytochemistry (ICC). View Larger

IL‑1 beta /IL‑1F2 in Human Peripheral Blood Mononuclear Cells. IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells using Goat Anti-Human IL-1 beta /IL-1F2 Polyclonal Antibody (Catalog # AB-201-NA) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Human IL‑1 beta / IL‑1F2 Antibody. View Larger

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Human IL‑1 beta / IL‑1F2 Antibody. Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta / IL-1F2 (50 pg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human IL-1 beta /IL-1F2 Poly-clonal Antibody (Catalog # AB-201-NA). The ND50 is typically 0.05-0.1 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

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Product Specific Notices

This product is covered by one or more of the following U.S. patents: 4,766,069, 5,510,462, 5,681,933, 4,762,914, 5,474,899, 5,789,185, 5,484,887, 5,122,459, 5,001,057, 5,077,219, 5,286,847.

Citations for Human IL-1 beta /IL-1F2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. MMP-9 and CXCL8/IL-8 Are Potential Therapeutic Targets in Epidermolysis Bullosa Simplex
    Authors: Thomas Lettner, Roland Lang, Alfred Klausegger, Stefan Hainzl, Johann W. Bauer, Verena Wally
    PLoS ONE
  2. AIM2 inflammasome mediates Arsenic-induced secretion of IL-1 beta and IL-18
    Authors: Mingfang Zhang, Yuanlin Qi, Hui Li, Jiajun Cui, Lu Dai, Jacqueline A. Frank et al.
    OncoImmunology
  3. Subcloning and characterization of highly metastatic cells derived from human esophageal squamous cell carcinoma KYSE150 cells by in vivo selection
    Authors: Masafumi Okuda, Jun Inoue, Naoto Fujiwara, Tatsuyuki Kawano, Johji Inazawa
    Oncotarget
  4. Cardiac Lymphatic Dysfunction Causes Drug-Eluting Stent–Induced Coronary Hyperconstricting Responses in Pigs In Vivo
    Authors: Hirokazu Amamizu, Yasuharu Matsumoto, Susumu Morosawa, Kazuma Ohyama, Hironori Uzuka, Michinori Hirano et al.
    Arteriosclerosis, Thrombosis, and Vascular Biology
  5. The Blockade of Tumoral IL1&beta-Mediated Signaling in Normal Colonic Fibroblasts Sensitizes Tumor Cells to Chemotherapy and Prevents Inflammatory CAF Activation
    Authors: NG Díaz-Marot, G Garcia-Vic, G Polcaro, M Bañuls, N Albert, A Villanueva, DG Molleví
    International Journal of Molecular Sciences, 2021-05-07;22(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells.
    Authors: Solanilla A, Dechanet J, El Andaloussi A, Dupouy M, Godard F, Chabrol J, Charbord P, Reiffers J, Nurden AT, Weksler B, Moreau JF, Ripoche J
    Blood, 2000-06-15;95(12):3758-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Human IL-1 beta /IL-1F2 Antibody
By Anonymous on 09/02/2022
Application: B/N Sample Tested: 293T human embryonic kidney cell line Species: Human