Human IL-1 beta /IL-1F2 Antibody Summary
Applications
Human IL-1 beta /IL-1F2 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-1 beta /IL-1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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IL‑1 beta /IL‑1F2 in Human PBMCs. IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB601) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Detection of Human IL‑1 beta /IL‑1F2 by Simple WesternTM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nm PMA and 10 ug/ml LPS for 24 hrs and 3 hrs, respectively, and loaded at 0.2 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 38 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Human IL‑1 beta /IL‑1F2 Antibody. Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta /IL-1F2 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB601). The ND50 is typically 0.05-0.2 µg/mL.
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Detection of Human IL-1 beta/IL-1F2 by ELISA Effect of A1AT on whole blood IL-1 beta release. IL-1 beta production in whole blood cultures in response to LPS (1.0 μg/ml) was performed in the presence of endogenous A1AT (i.e., undiluted) or exogenously added A1AT (2 mg/ml) in blood diluted 1:32 with RPMI. Whole blood cultures were incubated for 18 h. After incubation, plasma supernatants were removed, and IL-1 beta quantified by ELISA and expressed as mean ± SD for three donors. The diluted sample result was corrected for the dilution. NS indicates no significant difference. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0117330), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse IL-1 beta/IL-1F2 by Immunohistochemistry Inflammatory response induced by HI injury and maternal SE. Representative images of immunofluorescence staining of inflammatory cytokines IL-1 beta, IL-6, and TNF alpha in the cerebral cortex (A) and the hippocampus (E). The immunofluorescence intensity of IL-1 beta (B,F), IL-6 (C,G), and TNF alpha (D,H) in the cerebral cortex and hippocampus. Results are presented as mean ± SEM. *P < 0.05, **P < 0.01, n = 4, analyzed by two-way ANOVA followed by post hoc Turkey tests. SH, from sham exposed dams with sham surgery; HI, hypoxic-ischemic injury; SE, from smoke exposed dams with sham surgery; HI + SE, from smoke exposed dams with hypoxic-ischemic injury. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35250486), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human IL-1 beta/IL-1F2 by Western Blot Effect of high glucose and IL-1 beta alone or in combination on the protein expressions of CD31, FSP1 and alpha -SMA in HAECs. a–d HAECs were incubated for 48 h with NG and HG. Mannitol was used as a control for hyperosmolarity. Representative western blots (a) and quantitative determinations of CD31, FSP1 and alpha -SMA protein levels (b–d) are presented. e–h HAECs were treated for 48 h with NG, HG, IL-1 beta (10 ng/ml)and HG in the presence of the IL-1 beta (10 ng/ml). Representative western blots (E) and quantitative determinations of CD31, FSP1 and alpha -SMA protein levels (f–h) are presented. The data are expressed as the mean ± SD. Experiments were repeated at least three times. NG normal glucose (5.5 mM), HG high glucose (30 mM), MN 5.5 mM glucose + 24.5 mM mannitol, IL-1 beta (10 ng/ml), HG + IL-1 beta : high glucose (30 mM) + IL-1 beta (10 ng/ml) *P < 0.05 vs. MN or NG, **P < 0.01 vs. NG, #P < 0.05 vs. HG Image collected and cropped by CiteAb from the following publication (https://www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human IL-1 beta/IL-1F2 by Immunocytochemistry/Immunofluorescence The influence of high glucose or IL-1 beta on immunofluorescence of CD31 and FSP1 in HAECs. Representative immunofluorescence images showing CD31 (green), FSP1 (red) labeling and DAPI (blue) stains nuclei. a Normal ECs monolayers displayed a cobble stone morphology. b A merge of the three images revealed some cells populations that acquired a spindle-shaped morphology and lost CD31 expression (white arrow). c HAECs exposure to IL-1 beta alone for 48 h acquired a spindle-shaped morphology. d High glucose and IL-1 beta in combination resulted in decreased CD31 (the left white arrow) and increased FSP1staining (the right arrow). a normal glucose (5.5 mM) group, b high glucose (30 mM) group for 48 h; c treatment with a normal glucose (5.5 mM) + IL-1 beta (10 ng/ml) treatment for 48 h, d treatment with a high glucose (30 mM) + IL-1 beta (10 ng/ml) treatment for 48 h. Scale bar, 75 μm Image collected and cropped by CiteAb from the following publication (https://www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human IL-1 beta/IL-1F2 by Western Blot Effects of PKC beta on high glucose induced IL-1 beta up-regulation. Confluent cultures of HAECs were exposed to NG, HG, PMA (30 nM) and HG in the presence of the selective PKC beta inhibitors (LY317615, 0.3 μM) for 48 h. Real-time PCR analyses showed mRNA expression of PKC beta and IL-1 beta (a, b). Representative western blots (c) and quantitative determinations of PKC beta and IL-1 beta (d, e) are presented. The data are expressed as the mean ± SD. Experiments were repeated at least three times. NG normal glucose (5.5 mM), HG high glucose (30 mM), PMA (30 nM): phorbol 12-myristate13-acetate; LY (0.3 uM): LY317615; *P < 0.05 vs.NG, **P < 0.01 vs. NG, #P < 0.05 vs. HG or PMA Image collected and cropped by CiteAb from the following publication (https://www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse IL-1 beta/IL-1F2 by Immunohistochemistry Inflammatory response induced by HI injury and maternal SE. Representative images of immunofluorescence staining of inflammatory cytokines IL-1 beta, IL-6, and TNF alpha in the cerebral cortex (A) and the hippocampus (E). The immunofluorescence intensity of IL-1 beta (B,F), IL-6 (C,G), and TNF alpha (D,H) in the cerebral cortex and hippocampus. Results are presented as mean ± SEM. *P < 0.05, **P < 0.01, n = 4, analyzed by two-way ANOVA followed by post hoc Turkey tests. SH, from sham exposed dams with sham surgery; HI, hypoxic-ischemic injury; SE, from smoke exposed dams with sham surgery; HI + SE, from smoke exposed dams with hypoxic-ischemic injury. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35250486), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human IL-1 beta/IL-1F2 by Immunocytochemistry/Immunofluorescence The influence of high glucose or IL-1 beta on immunofluorescence of CD31 and FSP1 in HAECs. Representative immunofluorescence images showing CD31 (green), FSP1 (red) labeling and DAPI (blue) stains nuclei. a Normal ECs monolayers displayed a cobble stone morphology. b A merge of the three images revealed some cells populations that acquired a spindle-shaped morphology and lost CD31 expression (white arrow). c HAECs exposure to IL-1 beta alone for 48 h acquired a spindle-shaped morphology. d High glucose and IL-1 beta in combination resulted in decreased CD31 (the left white arrow) and increased FSP1staining (the right arrow). a normal glucose (5.5 mM) group, b high glucose (30 mM) group for 48 h; c treatment with a normal glucose (5.5 mM) + IL-1 beta (10 ng/ml) treatment for 48 h, d treatment with a high glucose (30 mM) + IL-1 beta (10 ng/ml) treatment for 48 h. Scale bar, 75 μm Image collected and cropped by CiteAb from the following publication (https://www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human IL-1 beta/IL-1F2 by Immunocytochemistry/Immunofluorescence The influence of high glucose or IL-1 beta on immunofluorescence of CD31 and FSP1 in HAECs. Representative immunofluorescence images showing CD31 (green), FSP1 (red) labeling and DAPI (blue) stains nuclei. a Normal ECs monolayers displayed a cobble stone morphology. b A merge of the three images revealed some cells populations that acquired a spindle-shaped morphology and lost CD31 expression (white arrow). c HAECs exposure to IL-1 beta alone for 48 h acquired a spindle-shaped morphology. d High glucose and IL-1 beta in combination resulted in decreased CD31 (the left white arrow) and increased FSP1staining (the right arrow). a normal glucose (5.5 mM) group, b high glucose (30 mM) group for 48 h; c treatment with a normal glucose (5.5 mM) + IL-1 beta (10 ng/ml) treatment for 48 h, d treatment with a high glucose (30 mM) + IL-1 beta (10 ng/ml) treatment for 48 h. Scale bar, 75 μm Image collected and cropped by CiteAb from the following publication (https://www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human IL-1 beta/IL-1F2 by Immunocytochemistry/Immunofluorescence The influence of high glucose or IL-1 beta on immunofluorescence of CD31 and FSP1 in HAECs. Representative immunofluorescence images showing CD31 (green), FSP1 (red) labeling and DAPI (blue) stains nuclei. a Normal ECs monolayers displayed a cobble stone morphology. b A merge of the three images revealed some cells populations that acquired a spindle-shaped morphology and lost CD31 expression (white arrow). c HAECs exposure to IL-1 beta alone for 48 h acquired a spindle-shaped morphology. d High glucose and IL-1 beta in combination resulted in decreased CD31 (the left white arrow) and increased FSP1staining (the right arrow). a normal glucose (5.5 mM) group, b high glucose (30 mM) group for 48 h; c treatment with a normal glucose (5.5 mM) + IL-1 beta (10 ng/ml) treatment for 48 h, d treatment with a high glucose (30 mM) + IL-1 beta (10 ng/ml) treatment for 48 h. Scale bar, 75 μm Image collected and cropped by CiteAb from the following publication (https://www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human IL-1 beta/IL-1F2 by Western Blot The influence of blocking IL-1 beta treatment on the protein expressions of CD31, FSP1, a-SMA, and IL-1 beta. (a–f) HAECs were incubated for 48 h with anti-IL-1 beta antibodies (1000 ng/ml) in the presence of NG or HG. (a1–f1) We performed gene-silencing experiments using transfection with siRNA specific for IL-1 beta. The protein expressions of IL-1 beta, CD31, FSP1 and alpha -SMA were assessed by western blotting. The data are expressed as the mean ± SD. Experiments were repeated at least three times. NG normal glucose (5.5 mM), HG high glucose (30 mM). Anti-IL-1 beta : anti-IL-1 beta antibodies (1000 ng/ml). *P < 0.05 vs. NG or anti-IL-1 beta, #P < 0.05 vs. HG or HG +Vehicle Image collected and cropped by CiteAb from the following publication (https://www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.
Product Datasheets
Product Specific Notices
This product is covered by one or more patents, including US Patent # 5,681,933.Citations for Human IL-1 beta /IL-1F2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Cervico-Vaginal Inflammatory Cytokine and Chemokine Responses to Two Different SIV Immunogens
Authors: Nikki P. L. Toledo, Hongzhao Li, Robert W. Omange, Tamara G. Dacoba, Jose Crecente-Campo, Dane Schalk et al.
Frontiers in Immunology
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Identification of Predictive Early Biomarkers for Sterile-SIRS after Cardiovascular Surgery.
Authors: Stoppelkamp S, Veseli K, Stang K, Schlensak C, Wendel H, Walker T
PLoS ONE, 2015-08-11;10(8):e0135527.
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Glucose Induces IL-1 alpha -Dependent Inflammation and Extracellular Matrix Proteins Expression and Deposition in Renal Tubular Epithelial Cells in Diabetic Kidney Disease
Authors: Talal Salti, Khaled Khazim, Rami Haddad, Salvatore Campisi-Pinto, Gil Bar-Sela, Idan Cohen
Frontiers in Immunology
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Mitochondrial CMPK2 mediates immunomodulatory and antiviral activities through IFN-dependent and IFN-independent pathways
Authors: Lai J, Wu D, Wu C et al.
iScience
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Vitamin D Induces Interleukin-1b Expression: Paracrine Macrophage Epithelial Signaling Controls M. tuberculosis Infection.
Authors: Verway M, Bouttier M, Wang TT et al.
PLoS Pathog.
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miR-146a-5p plays an essential role in the aberrant epithelial-fibroblast cross-talk in COPD
Authors: ET Osei, L Florez-Sam, H Tasena, A Faiz, JA Noordhoek, W Timens, DS Postma, TL Hackett, IH Heijink, CA Brandsma
Eur. Respir. J., 2017-05-25;49(5):.
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Inflammasome priming is similar for francisella species that differentially induce inflammasome activation.
Authors: Ghonime MG, Mitra S, Eldomany RA et al.
PLoS ONE.
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The Elevated Inflammatory Status of Neutrophils Is Related to In-Hospital Complications in Patients with Acute Coronary Syndrome and Has Important Prognosis Value for Diabetic Patients
Authors: Barbu, E;Mihaila, AC;Gan, AM;Ciortan, L;Macarie, RD;Tucureanu, MM;Filippi, A;Stoenescu, AI;Petrea, SV;Simionescu, M;Balanescu, SM;Butoi, E;
International journal of molecular sciences
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Tumor Necrosis Factor and Interleukin-1 beta Upregulate NRP2 Expression and Promote SARS-CoV-2 Proliferation
Authors: Ishitoku, M;Mokuda, S;Araki, K;Watanabe, H;Kohno, H;Sugimoto, T;Yoshida, Y;Sakaguchi, T;Masumoto, J;Hirata, S;Sugiyama, E;
Viruses
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Toxoplasma gondii profilin induces NLRP3 activation and IL-1beta production/secretion in THP-1�cells
Authors: H Pazoki, H Mirjalali, M Niyyati, SJ Seyed Taba, N Mosaffa, S Shahrokh, H Asadzadeh, A Kupz, MR Zali
Microbial pathogenesis, 2023-04-18;180(0):106120.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Dual-Enhanced Plasmonic Biosensing for Point-of-Care Sepsis Detection
Authors: Lip Ket Chin, Jun-Yeong Yang, Benjamin Chousterman, Sunghoon Jung, Do-Geun Kim, Dong-Ho Kim et al.
ACS Nano
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Toxoplasma gondii profilin induces NLRP3 activation and IL-1beta production/secretion in THP-1�cells
Authors: H Pazoki, H Mirjalali, M Niyyati, SJ Seyed Taba, N Mosaffa, S Shahrokh, H Asadzadeh, A Kupz, MR Zali
Microbial pathogenesis, 2023;180(0):106120.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Therapeutic Effects of Live Lactobacillus plantarum GKD7 in a Rat Model of Knee Osteoarthritis
Authors: YY Lin, SL Chang, SC Liu, D Achudhan, YS Tsai, SW Lin, YL Chen, CC Chen, JW Chang, YC Fong, SL Hu, CH Tang
Nutrients, 2022-08-01;14(15):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Oral Administration of Clostridium butyricum GKB7 Ameliorates Signs of Osteoarthritis in Rats
Authors: SL Chang, YY Lin, SC Liu, YS Tsai, SW Lin, YL Chen, CC Chen, CY Ko, HT Chen, WC Chen, CH Tang
Cells, 2022-07-11;11(14):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
The central inflammasome adaptor protein ASC activates the inflammasome after transition from a soluble to an insoluble state
Authors: ER Prather, MA Gavrilin, MD Wewers
The Journal of Biological Chemistry, 2022-05-11;0(0):102024.
Species: Human
Sample Types: Cell Lysates
Applications: ELISA (Capture) -
Pro-Inflammatory Cytokines Trigger the Overexpression of Tumour-Related Splice Variant RAC1B in Polarized Colorectal Cells
Authors: JFS Pereira, C Bessa, P Matos, P Jordan
Cancers, 2022-03-09;14(6):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Maternal Cigarette Smoke Exposure Exaggerates the Behavioral Defects and Neuronal Loss Caused by Hypoxic-Ischemic Brain Injury in Female Offspring
Authors: Taida Huang, Xiaomin Huang, Hui Li, Junhua Qi, Nan Wang, Yi Xu et al.
Frontiers in Cellular Neuroscience
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1,25-Dihydroxyvitamin D3 attenuates IL-1beta secretion by suppressing NLRP1 inflammasome activation by upregulating the NRF2-HO-1 pathway in epidermal keratinocytes
Authors: T Nakajo, T Katayoshi, N Kitajima, K Tsuji-Nait
Redox Biology, 2021-12-01;48(0):102203.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Machine learning identifies molecular regulators and therapeutics for targeting SARS‐CoV2‐induced cytokine release
Authors: Marina Chan, Siddharth Vijay, John McNevin, M Juliana McElrath, Eric C Holland, Taranjit S Gujral
Molecular Systems Biology
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Tumor‐associated macrophages promote the metastasis and growth of non‐small‐cell lung cancer cells through NF‐ kappa B/PP2Ac‐positive feedback loop
Authors: Zhan‐Wen Liang, Xin‐Xin Ge, Meng‐Dan Xu, Hualong Qin, Meng‐Yao Wu, Meng Shen et al.
Cancer Science
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Non-canonical roles of caspase-4 and caspase-5 in heme driven- IL-1 beta release and cell death
Authors: Beatriz E. Bolívar, Alexandra N. Brown-Suedel, Brittany A. Rohrman, Chloé I. Charendoff, Vanda Yazdani, John D. Belcher et al.
The Journal of Immunology
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Elevated Glucose Levels Favor SARS-CoV-2 Infection and Monocyte Response through a HIF-1 alpha /Glycolysis-Dependent Axis
Authors: Ana Campos Codo, Gustavo Gastão Davanzo, Lauar de Brito Monteiro, Gabriela Fabiano de Souza, Stéfanie Primon Muraro, João Victor Virgilio-da-Silva et al.
Cell Metabolism
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Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi�strains unveil a role for fibroblasts in neutrophil recruitment and activation
Authors: AER Oliveira, MCA Pereira, AT Belew, LRP Ferreira, LMN Pereira, EGA Neves, MDCP Nunes, BA Burleigh, WO Dutra, NM El-Sayed, RT Gazzinelli, SMR Teixeira
PLoS Pathog., 2020-08-18;16(8):e1008781.
Species: Human
Sample Types: Cell Lysates, Protein
Applications: Western Blot -
The IL1beta-IL1R signaling is involved in the stimulatory effects triggered by hypoxia in breast cancer cells and cancer-associated fibroblasts (CAFs)
Authors: R Lappano, M Talia, F Cirillo, DC Rigiraccio, D Scordamagl, R Guzzi, AM Miglietta, EM De Frances, A Belfiore, AH Sims, M Maggiolini
J. Exp. Clin. Cancer Res., 2020-08-10;39(1):153.
Species: Human
Sample Types: Cell Culture Lysates, Cell Lysates
Applications: Western Blot -
Anti-Inflammatory Performance of Lactose-Modified Chitosan and Hyaluronic Acid Mixtures in an In Vitro Macrophage-Mediated Inflammation Osteoarthritis Model
Authors: E Tarricone, E Mattiuzzo, E Belluzzi, R Elia, A Benetti, R Venerando, V Vindigni, P Ruggieri, P Brun
Cells, 2020-05-26;9(6):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Teneligliptin prevents doxorubicin-induced inflammation and apoptosis in H9c2 cells
Authors: W Peng, D Rao, M Zhang, Y Shi, J Wu, G Nie, Q Xia
Arch. Biochem. Biophys., 2019-12-24;0(0):108238.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Inhibition of IRE1 alpha RNase activity reduces NLRP3 inflammasome assembly and processing of pro-IL1 beta
Authors: Aaron Talty, Shane Deegan, Mila Ljujic, Katarzyna Mnich, Serika D. Naicker, Dagmar Quandt et al.
Cell Death & Disease
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Heterogeneous GM-CSF signaling in macrophages is associated with control of Mycobacterium tuberculosis
Authors: BD Bryson, TR Rosebrock, FG Tafesse, CY Itoh, A Nibasumba, GH Babunovic, B Corleis, C Martin, C Keegan, P Andrade, S Realegeno, D Kwon, RL Modlin, SM Fortune
Nat Commun, 2019-05-27;10(1):2329.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Structural Modification of the Antidepressant Mianserin Suggests That Its Anti-inflammatory Activity May Be Independent of 5-Hydroxytryptamine Receptors
Authors: Sandra Sacre, Albert Jaxa-Chamiec, Caroline M. R. Low, Giselle Chamberlain, Cathy Tralau-Stewart
Frontiers in Immunology
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Exposure to wild-type AAV drives distinct capsid immunity profiles in humans
Authors: K Kuranda, P Jean-Alpho, C Leborgne, R Hardet, F Collaud, S Marmier, H Costa Verd, G Ronzitti, P Veron, F Mingozzi
J. Clin. Invest., 2018-10-22;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
DPP8/DPP9 inhibitor-induced pyroptosis for treatment of acute myeloid leukemia
Authors: DC Johnson, CY Taabazuing, MC Okondo, AJ Chui, SD Rao, FC Brown, C Reed, E Peguero, E de Stanchi, A Kentsis, DA Bachovchin
Nat. Med., 2018-07-02;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Targeting the miR-665-3p-ATG4B-autophagy axis relieves inflammation and apoptosis in intestinal ischemia/reperfusion
Authors: Z Li, G Wang, D Feng, G Zu, Y Li, X Shi, Y Zhao, H Jing, S Ning, W Le, J Yao, X Tian
Cell Death Dis, 2018-05-01;9(5):483.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Saturated Fatty Acids Undergo Intracellular Crystallization and Activate the NLRP3 Inflammasome in Macrophages
Authors: T Karasawa, A Kawashima, F Usui-Kawan, S Watanabe, H Kimura, R Kamata, K Shirasuna, Y Koyama, A Sato-Tomit, T Matsuzaka, H Tomoda, SY Park, N Shibayama, H Shimano, T Kasahara, M Takahashi
Arterioscler. Thromb. Vasc. Biol., 2018-02-08;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Calcium phosphate particles stimulate interleukin-1 beta release from human vascular smooth muscle cells: A role for spleen tyrosine kinase and exosome release
Authors: Yana Dautova, Alexander N. Kapustin, Kevin Pappert, Matthias Epple, Hanneke Okkenhaug, Simon J. Cook et al.
Journal of Molecular and Cellular Cardiology
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ANKRD1 regulates innate immune responses against herpes simplex virus 1: A potential role in eczema herpeticum
Authors: L Bin, X Li, B Richers, JE Streib, JW Hu, P Taylor, DYM Leung
J. Allergy Clin. Immunol., 2018-01-31;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralizing -
Innate activation of human primary epithelial cells broadens the host response to Mycobacterium tuberculosis in the airways
Authors: AK Reuschl, MR Edwards, R Parker, DW Connell, L Hoang, A Halliday, H Jarvis, N Siddiqui, C Wright, S Bremang, SM Newton, P Beverley, RJ Shattock, OM Kon, A Lalvani
PLoS Pathog., 2017-09-01;13(9):e1006577.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells
Authors: Y Fan, X Zhang, L Yang, J Wang, Y Hu, A Bian, J Liu, J Ma
Mol Med Rep, 2017-08-11;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Interleukin-1 beta mediates high glucose induced phenotypic transition in human aortic endothelial cells
Authors: Dong-Dong Zhu, Ri-Ning Tang, Lin-Li Lv, Yi Wen, Hong Liu, Xiao-Liang Zhang et al.
Cardiovascular Diabetology
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Interleukin-1 alpha drives the dysfunctional cross-talk of the airway epithelium and lung fibroblasts in COPD
Authors: Emmanuel T. Osei, Jacobien A. Noordhoek, Tillie L. Hackett, Anita I.R. Spanjer, Dirkje S. Postma, Wim Timens et al.
European Respiratory Journal
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Offloading treatment is linked to activation of proinflammatory cytokines and start of bone repair and remodeling in Charcot arthropathy patients
Authors: Agnetha Folestad, Martin Ålund, Susanne Asteberg, Jesper Fowelin, Ylva Aurell, Jan Göthlin et al.
Journal of Foot and Ankle Research
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Cytokine detection and simultaneous assessment of rheumatoid factor interference in human serum and synovial fluid using high-sensitivity protein arrays on plasmonic gold chips
Authors: Manfè Valentina, Fleckner Jan, Nørby Lisby Peder, Zhang Bo, Dai Hongjie, Keller Pernille
BMC Biotechnology
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Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells.
Authors: Sommer A, Fabri M
PLoS ONE, 2015-06-24;10(6):e0130395.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Alpha 1-antitrypsin does not inhibit human monocyte caspase-1.
Authors: Rahman M, Mitra S, Sarkar A, Wewers M
PLoS ONE, 2015-02-06;10(2):e0117330.
Species: Human
Sample Types: Plasma
Applications: ELISA Development (Capture) -
Small molecules dorsomorphin and LDN-193189 inhibit myostatin/GDF8 signaling and promote functional myoblast differentiation.
Authors: Horbelt D, Boergermann J, Chaikuad A, Alfano I, Williams E, Lukonin I, Timmel T, Bullock A, Knaus P
J Biol Chem, 2014-11-03;290(6):3390-404.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Inflammasome priming by lipopolysaccharide is dependent upon ERK signaling and proteasome function.
Authors: Ghonime M, Shamaa O, Das S, Eldomany R, Fernandes-Alnemri T, Alnemri E, Gavrilin M, Wewers M
J Immunol, 2014-03-12;192(8):3881-8.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture) -
IL-17C regulates the innate immune function of epithelial cells in an autocrine manner.
Authors: Ramirez-Carrozzi V, Sambandam A, Luis E
Nat. Immunol., 2011-10-12;12(12):1159-66.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis.
Authors: Hong YS, Moon SJ, Moon S, Joo YB, Joo Y, Jeon CH, Jeon C, Cho ML, Ju JH, Oh HJ, Heo YJ, Heo Y, Park SH, Kim HY, Min JK
J Korean Med Sci, 2011-09-01;26(9):1132-9.
Species: Human
Sample Types: Synovial Fluid
Applications: ELISA Development -
Correlations between both the expression levels of inflammatory mediators and growth factor in medial perimeniscal synovial tissue and the severity of medial knee osteoarthritis
Authors: Liang Ning, Muneaki Ishijima, Haruka Kaneko, Hidetake Kurihara, Eri Arikawa-Hirasawa, Mitsuaki Kubota et al.
International Orthopaedics
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Staphylococcus aureus stimulates neutrophil targeting chemokine expression in keratinocytes through an autocrine IL-1alpha signaling loop
Authors: Florina Olaru, Liselotte E. Jensen
Journal of Investigative Dermatology
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Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
J. Immunol., 2009-10-07;183(9):5799-806.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation, 2009-06-27;87(12):1814-20.
Species: Human
Sample Types: Urine
Applications: Antibody Array Development -
MicroRNAs miR-146a/b negatively modulate the senescence-associated inflammatory mediators IL-6 and IL-8
Authors: Dipa Bhaumik, Gary K. Scott, Shiruyeh Schokrpur, Christopher K. Patil, Arturo V. Orjalo, Francis Rodier et al.
ImpactJ
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Endogenous IL-32 controls cytokine and HIV-1 production.
Authors: Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA
J. Immunol., 2008-07-01;181(1):557-65.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Electrochemiluminescent Assay -
Bacterial vaginosis, not HIV, is primarily responsible for increased vaginal concentrations of proinflammatory cytokines.
Authors: Mitchell CM, Balkus J, Agnew KJ, Cohn S, Luque A, Lawler R, Coombs RW, Hitti JE
AIDS Res. Hum. Retroviruses, 2008-05-01;24(5):667-71.
Species: Human
Sample Types: Vaginal Fluid
Applications: ELISA Development -
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Authors: Pfleger C, Schloot N, ter Veld F
J. Immunol. Methods, 2007-10-22;329(1):214-8.
Species: Human
Sample Types: Serum
Applications: Luminex Development -
Ultrasensitive flow-based immunoassays using single-molecule counting.
Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
Clin. Chem., 2007-09-21;53(11):1990-5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production.
Authors: Emsley HC, Smith CJ, Gavin CM
BMC Neurol, 2007-02-28;7(0):5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood, 2007-02-08;109(10):4272-9.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
AIDS, 2007;21(10):1263-72.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
HIV-1-infected and/or immune activated macrophages regulate astrocyte SDF-1 production through IL-1beta.
Authors: Peng H, Erdmann N, Whitney N, Dou H, Gorantla S, Gendelman HE, Ghorpade A, Zheng J
Glia, 2006-11-01;54(6):619-29.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Changes in periodontal and rheumatological conditions after 2 years in patients with juvenile idiopathic arthritis.
Authors: Miranda LA, Braga F, Fischer RG, Sztajnbok FR, Figueredo CM, Gustafsson A
J. Periodontol., 2006-10-01;77(10):1695-700.
Species: Human
Sample Types:
Applications: ELISA Development -
Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
Authors: Phillips TM
Electrophoresis, 2004-06-01;25(10):1652-9.
Species: Human
Sample Types: Serum
Applications: Affinity Chromatography -
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Authors: Copeland S, Siddiqui J, Remick D
J. Immunol. Methods, 2004-01-01;284(1):99-106.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Array Development
FAQs
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Can pro-IL-1 beta be detected by Catalog #s AF-201-NA and MAB601 ?
The amino acid sequence present in mature IL-1 beta (Ala117-Ser269) is also present in pro IL-1 beta. So in theory, both Catalog #s AF-201-NA and MAB601 should detect the pro form of IL-1 beta. The results would depend on whether the pro form is abundantly present in the sample type being evaluated.
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Average Rating: 4 (Based on 4 Reviews)
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