Home / IL-18 R alpha/IL-1 R5 / Human IL-18 R alpha /IL-1 R5 Antibody

Human IL-18 R alpha /IL-1 R5 Antibody

Catalog #: AF840
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF840
AF840-SP
IL‑18 R alpha /IL‑1 R5 in Human Skin.
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Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-18 R alpha /IL-1 R5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑18 R alpha /IL-1 R5 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse IL‑18 R alpha is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5
Glu20-Arg329
Accession # Q13478
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑18 R alpha /IL‑1 R5 Fc Chimera (Catalog # 816-LR)
Flow Cytometry
2.5 µg/106 cells
Human CD3+ T cells treated with PHA and Recombinant Human IL‑2 (Catalog # 202-IL)
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 40 ng/mL Recombinant Human IL‑18/IL‑1F4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry IL‑18 Ra/IL‑1 R5 antibody in Human Skin by Immunohistochemistry (IHC-Fr). View Larger

IL‑18 R alpha /IL‑1 R5 in Human Skin. IL‑18 Ra/IL‑1 R5 was detected in immersion fixed frozen sections of human skin using Goat Anti-Human IL‑18 Ra/IL‑1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF840) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of keratinocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Neutralization IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutralization by Human IL‑18 R alpha /IL‑1 R5 Antibody. View Larger

IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutralization by Human IL‑18 R alpha /IL‑1 R5 Antibody. Recombinant Human IL‑18/IL‑1F4 stimulates IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line, in a dose-dependent manner (orange line), as measured by the Human IFN‑ gamma Quantikine ELISA Kit (Catalog # DIF50C). IFN‑ gamma secretion elicited by IL‑18/IL‑1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑18 Ra/IL‑1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF840). The ND50 is typically 0.1-0.4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-18 R alpha/IL-1 R5

Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immuno-regulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL‑18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa residues. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate, and placenta.

References
  1. Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
  2. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
  3. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
Long Name
Interleukin 18 Receptor alpha
Entrez Gene IDs
8809 (Human); 16182 (Mouse)
Alternate Names
CD218a; CD218a;CDw218a;IL18R1;IL18RA;IL-18Ralpha;IL-18R-alpha;IL18Ralpha2;IL1RRP;IL-1Rrp;Interleukin-18 receptor 1; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL18R alpha; IL18R1; IL-18Ra

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Citations for Human IL-18 R alpha /IL-1 R5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Chronic hepatitis delta virus infection leads to functional impairment and severe loss of MAIT cells
    Authors: Joana Dias, Julia Hengst, Tiphaine Parrot, Edwin Leeansyah, Sebastian Lunemann, David F.G. Malone et al.
    Journal of Hepatology
  2. Interleukin-18 enhances IL-18R/Nox1 binding, and mediates TRAF3IP2-dependent smooth muscle cell migration. Inhibition by simvastatin.
    Authors: Valente AJ, Yoshida T, Izadpanah R et al.
    Cell Signal.
  3. Role of mucosal-associated invariant T cells dynamics in pathogenesis of Sjögren syndrome
    Authors: Chauffier, J;Berger de Gallardo, H;Chevalier, MF;Kante, A;Lambert, M;Cabrol, X;Aldersons, E;Mouly, S;Champion, K;Amador-Borrero, B;Burlacu, R;Bigot, W;Adle-Biassete, H;Kaci, R;Selvanadin, A;Cohen-Solal, M;Coudert, A;Caillat-Zucman, S;Sène, D;Comarmond, C;
    Scientific reports
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  4. Novel PROTAC probes targeting FOSL1 degradation to eliminate head and neck squamous cell carcinoma cancer stem cells
    Authors: Zaman, SU;Pagare, PP;Huang, B;Rilee, G;Ma, Z;Zhang, Y;Li, J;
    Bioorganic chemistry
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma
    Sci Rep, 2016-05-26;6(0):26757.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. MAIT cells reside in the female genital mucosa and are biased towards IL-17 and IL-22 production in response to bacterial stimulation
    Authors: A Gibbs, E Leeansyah, A Introini, D Paquin-Pro, K Hasselrot, E Andersson, K Broliden, JK Sandberg, A Tjernlund
    Mucosal Immunol, 2016-04-06;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Loss of methylation at the IFNG promoter and CNS-1 is associated with the development of functional IFN-gamma memory in human CD4(+) T lymphocytes.
    Authors: Dong J, Chang H, Ivascu C, Qian Y, Rezai S, Okhrimenko A, Cosmi L, Maggi L, Eckhardt F, Wu P, Sieper J, Alexander T, Annunziato F, Gossen M, Li J, Radbruch A, Thiel A
    Eur J Immunol, 2013-03-01;43(3):793-804.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Human MAIT cells are xenobiotic-resistant, tissue-targeted, CD161hi IL-17-secreting T cells.
    Authors: Dusseaux M, Martin E, Serriari N, Peguillet I, Premel V, Louis D, Milder M, Le Bourhis L, Soudais C, Treiner E, Lantz O
    Blood, 2010-11-17;117(4):1250-9.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  9. Differentiation of human SH-SY5Y neuroblastoma cells by all-trans retinoic acid activates the interleukin-18 system.
    Authors: Sallmon H, Hoene V, Weber SC, Dame C
    J. Interferon Cytokine Res., 2010-02-01;30(2):55-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Caspase-1-processed interleukins in hyperoxia-induced cell death in the developing brain.
    Authors: Felderhoff-Mueser U, Sifringer M, Polley O, Dzietko M, Leineweber B, Mahler L, Baier M, Bittigau P, Obladen M, Ikonomidou C, Buhrer C
    Ann. Neurol., 2005-01-01;57(1):50-9.
    Species: Rat
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot

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