Human IL-18/IL-1F4 Antibody

Catalog # Availability Size / Price Qty
D043-3
Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (1)

Human IL-18/IL-1F4 Antibody Summary

Species Reactivity
Human
Specificity
This antibody reacts with human IL-18 on Western blotting.
Source
Monoclonal Mouse IgG1 Clone # 25-2G
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human IL-18/IL-1F4
Formulation
This antibody is in lyophilized form. After reconstitution, IgG concentration should be 1 mg/mL in PBS (pH 7.2)/1% sucrose. Contains no preservatives.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 μg/mL for chemiluminescencedetection system
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Prepare a stock solution by dissolving the lyophilized antibody with 100 μL of distilled water.
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Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for one year from the date of purchase when stored at 4°C. After reconstitution, avoid repeated freezing and thawing. For storage, prepare appropriate aliquots and freeze at -20°C.

Background: IL-18/IL-1F4

Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.

References
  1. McCurnin, D., et al., Pediatrics 121, 945-956 (2008)
  2. Haveman, L. M., et al., Int. Immunol. 18, 1521-1529 (2006)
  3. Shigehara, K., et al., J. Immunol. 166, 642-649 (2001)
  4. Dao, T., et al. Cell Immunol. 173, 230-235 (1996)
  5. Micallef, M., et al. Eur. J. Immunol. 26, 1647-1651 (1996)
  6. Ushio, S., et al. J. Immunol. 156, 4274-4279 (1996)
  7. Okamura, H., et al. Nature 378, 88-91 (1995)
Long Name
Interleukin 18
Entrez Gene IDs
3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names
Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18

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Citations for Human IL-18/IL-1F4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. AIM2 inflammasome mediates Arsenic-induced secretion of IL-1 beta and IL-18
    Authors: Mingfang Zhang, Yuanlin Qi, Hui Li, Jiajun Cui, Lu Dai, Jacqueline A. Frank et al.
    OncoImmunology
  2. Interleukin-18 enhances IL-18R/Nox1 binding, and mediates TRAF3IP2-dependent smooth muscle cell migration. Inhibition by simvastatin.
    Authors: Valente AJ, Yoshida T, Izadpanah R et al.
    Cell Signal.
  3. The Nox1/4 Dual Inhibitor GKT137831 or Nox4 Knockdown Inhibits Angiotensin-II-Induced Adult Mouse Cardiac Fibroblast Proliferation and Migration. AT1 Physically Associates With Nox4
    Authors: Naveen K. Somanna, Anthony J. Valente, Maike Krenz, William P. Fay, Patrice Delafontaine, Bysani Chandrasekar
    Journal of Cellular Physiology
  4. Surface cysteine to serine substitutions in IL-18 reduce aggregation and enhance activity
    Authors: J Saetang, N Roongsawan, S Sangkhatha, SP Voravuthik, N Sangkaew, N Prompat, T Srichana, V Tipmanee
    PeerJ, 2022-07-05;10(0):e13626.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells
    Authors: Y Fan, X Zhang, L Yang, J Wang, Y Hu, A Bian, J Liu, J Ma
    Mol Med Rep, 2017-08-11;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Resveratrol blocks interleukin-18-EMMPRIN cross-regulation and smooth muscle cell migration.
    Authors: Venkatesan B, Valente AJ, Reddy VS, Siwik DA, Chandrasekar B
    Am. J. Physiol. Heart Circ. Physiol., 2009-06-26;297(2):H874-86.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Clinical improvement in chronic plaque-type psoriasis lesions after narrow-band UVB therapy is accompanied by a decrease in the expression of IFN-gamma inducers -- IL-12, IL-18 and IL-23.
    Authors: Piskin G, Tursen U, Sylva-Steenland RM, Bos JD, Teunissen MB
    Exp. Dermatol., 2004-12-01;13(12):764-72.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. Cytokine profile of the rheumatoid nodule suggests that it is a Th1 granuloma.
    Authors: Hessian PA, Highton J, Kean A, Sun CK, Chin M
    Arthritis Rheum., 2003-02-01;48(2):334-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Histone deacetyltransferase inhibitors Trichostatin A and Mocetinostat differentially regulate MMP9, IL-18 and RECK expression, and attenuate Angiotensin II-induced cardiac fibroblast migration and proliferation
    Authors: Naveen K Somanna, Anthony J Valente, Maike Krenz, Kerry S McDonald, Yusuke Higashi, Makoto Noda et al.
    Hypertension Research

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Reviews for Human IL-18/IL-1F4 Antibody

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Human IL-18/IL-1F4 Antibody
By Anonymous on 10/31/2017
Application: ELISA Sample Tested: CHO Chinese hamster ovary cell line Species: Hamster