Human IL-17/IL-17A Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB3171R-100
MAB3171R-SP
MAB3171R-500
MAB3171R-01M
Detection of Human IL‑17/IL‑17A by Western Blot.
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Human IL-17/IL-17A Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-17 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer and no cross-reactivity with rhIL-17F, recombinant mouse IL-17, and recombinant rat IL-17A is observed.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 41802R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E.coli-derived recombinant human IL-17
Ile20-Ala155
Accession # Q16552
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IL-17/IL-17A antibody by Western Blot. View Larger

Detection of Human IL‑17/IL‑17A by Western Blot. Western blot shows lysates of human CD4+T cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-17/IL-17A Monoclonal Antibody (Catalog # MAB3171R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-17/IL-17A at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human IL-17/IL-17A antibody by Western Blot. View Larger

Detection of Human IL‑17/IL‑17A by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-17/IL-17A. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-17/IL-17A Monoclonal Antibody (Catalog # MAB3171R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for IL-17/IL-17A at approximately 14-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intracellular Staining by Flow Cytometry Detection of IL-17/IL-17A antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of IL‑17/IL‑17A in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 250 ng/mL calcium ionomycin for 16 hours, then stained with Mouse Anti-Human IL-17/IL-17A Monoclonal Antibody (Catalog # MAB3171R, dark orange filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17/IL-17A

Interleukin-17 (IL-17) is a pro-inflammatory cytokine secreted by activated T cells. It is the prototype member of the IL-17 family that also includes IL-17B, C, D, E, and F.

Long Name
Interleukin 17
Entrez Gene IDs
3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

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