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Human IL-1 RII Antibody

Catalog #: MAB263
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB263
MAB263-SP
IL‑1 RII Inhibition of IL‑1 beta /IL‑1F2‑dependent Cell Proliferation and Neutralization by Human IL‑1 RII Antibody.
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Citations (3)
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Human IL-1 RII Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-1 RII in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL‑18 R, and recombinant mouse IL-18 R is observed.
Source
Monoclonal Mouse IgG2a Clone # 32437
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑1 RII (Catalog # 263-2R) under non-reducing conditions only
Immunocytochemistry
8-25 µg/mL
Immersion fixed HDLM‑2 human Hodgkin's lymphoma cells (positive) and absent in K562 human chronic myelogenous leukemia cells (negative)
Neutralization
Measured by its ability to neutralize IL‑1 RII-mediated inhibition of proliferation in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND50) is typically 2-6 µg/mL in the presence of 2 µg/mL Recombinant Human IL‑1 RII, 50 pg/mL Recombinant Human IL‑1 beta /IL‑1F2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IL‑1 RII Inhibition of IL‑1 beta /IL‑1F2‑dependent Cell Proliferation and Neutralization by Human IL‑1 RII Antibody. View Larger

IL‑1 RII Inhibition of IL‑1 beta /IL‑1F2‑dependent Cell Proliferation and Neutralization by Human IL‑1 RII Antibody. Recombinant Human IL-1 RII (263-2R) inhibits Recombinant Human IL-1 beta / IL-1F2 (201-LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) activity elicited by Recombinant Human IL-1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 RII Monoclonal Antibody (Catalog # MAB263). The ND50 is typically 2-6 µg/mL.

Immunocytochemistry View Larger

Detection of IL‑1 RII in HDLM-2 (positive) and K562 (negative). IL‑1 RII was detected in immersion fixed HDLM‑2 human Hodgkin’s lymphoma cells (positive) and absent in K562 human chronic myelogenous leukemia cells (negative) using Mouse Anti-Human IL‑1 RII Monoclonal Antibody (Catalog # MAB263) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Flow Cytometry View Larger

Detection of IL‑1 RII in HDLM-2 cells by Flow Cytometry HDLM-2 cells were stained with Mouse Anti-Human IL‑1 RII Monoclonal Antibody (Catalog # MAB263, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IL-1 RII

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts and endothelial cells. IL-1 receptor type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex.

An IL-1 receptor accessory protein that can heterodimerize with the type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling type I receptor complex.

References
  1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.
Long Name
Interleukin 1 Receptor II
Entrez Gene IDs
7850 (Human); 16178 (Mouse)
Alternate Names
beta; CD121b antigen; CD121b; IL-1 R beta; IL-1 RII; IL1R2; IL1RBCD121 antigen-like family member B; IL-1R-beta; IL1RII; IL-1RII; IL-1RT2; IL-1RT-2; interleukin 1 receptor, type II; Interleukin-1 receptor beta; MGC47725

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Citations for Human IL-1 RII Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1 beta -dependent response of Th17 cells
    Authors: Dong Hyun Kim, Hee Young Kim, Sunjung Cho, Su-Jin Yoo, Won-Ju Kim, Hye Ran Yeon et al.
    eLife
  2. Isolation and characterization of human trophoblast side-population (SP) cells in primary villous cytotrophoblasts and HTR-8/SVneo cell line.
    Authors: Takao T, Asanoma K, Kato K
    PLoS ONE, 2011-07-07;6(7):e21990.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Cell Selection, ICC, Western Blot
  3. Immunolocalization of interleukin-1 receptors in the sarcolemma and nuclei of skeletal muscle in patients with idiopathic inflammatory myopathies.
    Authors: Grundtman C, Salomonsson S, Dorph C, Bruton J, Andersson U, Lundberg IE
    Arthritis Rheum., 2007-02-01;56(2):674-87.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr

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