Human IFN-kappa Antibody

Catalog # Availability Size / Price Qty
MAB10247-100
MAB10247-SP
Human IFN‑ kappa  ELISA Standard Curve.
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Human IFN-kappa Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN‑ kappa in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 1009712
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IFN‑ kappa
Leu28-Lys207
Accession # Q90W0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human IFN‑ kappa Monoclonal Antibody (Catalog # MAB102472).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Human IFN‑ kappa ELISA Standard Curve. Recombinant Human IFN-kappa protein was serially diluted 2-fold and captured by Mouse Anti-Human IFN-kappa Monoclonal Antibody (Catalog # MAB10247) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IFN-kappa Monoclonal Antibody (Catalog # MAB102472) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

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Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-kappa

Interferon kappa, also known as IFN-kappa, is a 207 aminoacids protein that in humans is encoded by the IFNK gene. IFN-kappa is a member of the type I interferon family which also includes IFN-alpha, -beta, -epsilon, and -omega. Type I interferons are a group of related glycoproteins that play an important role in host defenses against viral infections. Human IFN-kappa has been detected in keratinocytes, monocytes, and monocyte-derived dendritic cells and is reported to have contact-dependent antiviral activity. Human papillomavirus (HPV) 16 oncogene expression, which is necessary for the development of cervical cancer, has been shown to down-regulate human IFN-kappa expression.

Long Name
Interferon kappa
Entrez Gene IDs
56832 (Human); 387510 (Mouse)
Alternate Names
IFNK; IFNkappa; IFN-kappa

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