Human IFN-gamma  R1/CD119 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB673P
Detection of IFN‑ gamma  R1/CD119 in Human Blood Monocytes by Flow Cytometry.
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Product Details
Citations (5)
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Human IFN-gamma  R1/CD119 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN-gamma R1 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant mouse IFN‑ gamma  R1, recombinant human (rh) IFN-gamma R2, or rhIL-10 R beta.
Source
Monoclonal Mouse IgG1 Clone # 92101
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IFN-gamma  R1
Glu18-Gly245
Accession # P15260.1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IFN‑? R1/CD119 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of IFN‑ gamma R1/CD119 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human IFN-gamma R1/CD119 PE-conjugated Monoclonal Antibody (Catalog # FAB673P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IFN-gamma R1/CD119

The high-affinity IFN-gamma  receptor complex is made up of two type I transmembrane proteins, IFN-gamma  R1 (IFN-gamma  R alpha ) and IFN-gamma  R2 (IFN-gamma  R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% amino acid sequence identity. IFN-gamma  R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma  R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma  R1 cDNA encodes a 489 amino acid (aa) residue protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma  R1 share 52% amino acid sequence identity and bind IFN-gamma in a species-specific manner. IFN-gamma  R1 is constitutively expressed in most cell types. Soluble IFN-gamma  R1 that binds IFN-gamma has been detected in biological fluids.

Long Name
Interferon gamma Receptor 1
Entrez Gene IDs
3459 (Human); 15979 (Mouse)
Alternate Names
AVP, type 2; CD119 antigen; CD119; FLJ45734; IFN-gamma R1; IFN-gamma receptor 1; IFNgammaR1; IFN-gamma-R1; IFNGR; IFNGR1; IFN-gR1; immune interferon receptor 1; interferon gamma receptor 1; interferon-gamma receptor alpha chain; type 2

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Citations for Human IFN-gamma  R1/CD119 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Regulation of PD-L1 expression in non–small cell lung cancer by interleukin-1 beta
    Authors: Aiko Hirayama, Kentaro Tanaka, Hirono Tsutsumi, Takayuki Nakanishi, Sho Yamashita, Shun Mizusaki et al.
    Frontiers in Immunology
  2. An ex vivo tumor fragment platform to dissect response to PD-1 blockade in cancer
    Authors: P Voabil, M de Bruijn, LM Roelofsen, SH Hendriks, S Brokamp, M van den Br, A Broeks, J Sanders, P Herzig, A Zippelius, CU Blank, KJ Hartemink, K Monkhorst, JBAG Haanen, TN Schumacher, DS Thommen
    Nature Medicine, 2021-07-08;27(7):1250-1261.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Function of multiple sclerosis-protective HLA class I alleles revealed by genome-wide protein-quantitative trait loci mapping of interferon signalling
    Authors: C Lundtoft, P Pucholt, J Imgenberg-, J Carlsson-A, ML Eloranta, AC Syvänen, G Nordmark, JK Sandling, I Kockum, T Olsson, L Rönnblom, N Hagberg
    PLoS Genet, 2020-10-26;16(10):e1009199.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Circulating microparticle subpopulations in systemic lupus erythematosus are affected by disease activity
    Authors: P López, J Rodríguez-, A Martínez-Z, L Caminal-Mo, A Suárez
    Int. J. Cardiol, 2017-03-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Systematic cytokine receptor profiling reveals GM-CSF as a novel TLR-independent activator of human plasmacytoid predendritic cells.
    Authors: Ghirelli C, Zollinger R, Soumelis V
    Blood, 2010-04-09;115(24):5037-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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