Human IFN-alpha 2/IFNA2 Antibody

Catalog #: MAB9345 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9345-100
MAB9345-SP

IFN‑ alpha 2/IFNA2 in HeLa Human Cell Line.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IFN-alpha 2/IFNA2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IFN-alpha 2/IFNA2 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IFNE, rhIFNB, rhIFNG, or recombinant cotton rat IFNA is observed. In sandwich ELISAs, less than 8% cross-reactivity with rhIFNA-7, rhIFNA-8, and rhIFNA-10 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 967314

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell line HEK-293-derived transfected with human IFN-alpha 2/IFNA2
Met1-Glu188
Accession # P01563

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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IFN‑ alpha 2/IFNA2 in HeLa Human Cell Line. IFN-a2/IFNA2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human IFN-a2/IFNA2 Monoclonal Antibody (Catalog # MAB9345) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha 2/IFNA2

Interferon-alpha 2 (IFNA2), also known as leukocyte interferon, is a type I interferon produced by macrophages, CD8 resting T cells, tonsillar NK cells, germinal center B cells, as well as epithelial cells, tumor cells and melanocytes. IFN alpha has at least nine different isoforms.

IFNA2 is a ligand for a cell surface receptor with two subunits, IFN-alpha R2 (ligand binding) and IFN-alpha R1 (ligand binding and signal transduction). IFNA2 has both antiviral and immunomodulatory activities on target cells. Intranasal administration has been shown to reduce viral load in subjects with the common cold. While IFNA2 is a prognostic factor for hepatocellular carcinoma, Interferon alpha 2b, has been used to treat melanoma, renal cell carcinoma, chronic myelogenous leukemia and hepatitis C.

Long Name

Interferon alpha 2

Entrez Gene IDs

3440 (Human); 15965 (Mouse); 298213 (Rat)

Alternate Names

alpha-2a interferon; Ifa2; IFNA; IFNA2; IFNA2a; IFNA2b; IFNA2c; IFNAA; IFNalpha 2; IFN-alpha 2; IFN-alpha-2; IFN-alphaA; INFA2; interferon alpha 2b; interferon alpha A; interferon alpha-2; Interferon alpha-A; interferon, alpha 2; LeIF A; MGC125764; MGC125765