Home / Iduronate 2-Sulfatase/IDS / Human Iduronate 2-Sulfatase/IDS Antibody

Human Iduronate 2-Sulfatase/IDS Antibody

Catalog #: AF2449
6 Citations Datasheet / COA / SDS
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AF2449
AF2449-SP
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Detection of Human Iduronate 2‑Sulfatase/IDS by Western Blot.
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Citations (6)
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Human Iduronate 2-Sulfatase/IDS Antibody Summary

Species Reactivity
Human
Specificity
Detects human IDS in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IDS is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IDS
Ser26-Pro550
Accession # P22304
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Iduronate 2-Sulfatase/IDS (Catalog # 2449-SU), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Iduronate 2-Sulfatase/IDS antibody by Western Blot. View Larger

Detection of Human Iduronate 2‑Sulfatase/IDS by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMCs). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Iduronate 2-Sulfatase/IDS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2449) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Iduronate 2-Sulfatase/IDS at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Iduronate 2-Sulfatase/IDS

As a member of the sulfatase family, IDS is required for the lysosomal degradation of the glycosaminoglycans (GAG) heparan sulfate and dermatan sulfate (2, 3). It hydrolyzes the 2‑sulfate group of the L-iduronate 2-sulfate units of the GAG. The IDS deficiency results in mucopolysaccharidosis II (MPS II or Hunter syndrome), an X-linked inborn error leading to lysosomal accumulation of the GAG and its excretion in urine. MPS II has a wide spectrum of clinical manifestations ranging from mild to severe. The deduced amino acid sequence of human IDS consists of a signal peptide (residues 1-25), a pro peptide (residues 26-33) and a mature chain (residues 34-550) that may be further processed into the 42 kDa chain (residues 34-455) and the 14 kDa chain (residues 456-550) (1). Recombinant human IDS corresponds to the single chain and has sulfatase activity described above.

References
  1. Wilson, P.J. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8531.
  2. Parenti, G. et al. (1997) Curr. Opin. Genet. & Dev. 7:386.
  3. Neufeld, E.F. and J. Muenzer (2001) in The Metabolic and Molecular Basis of Inherited Disease, Scriver, C.R. et al. (eds.) pp. 3421-3452, New York, McGraw-Hill.
Entrez Gene IDs
3423 (Human); 15931 (Mouse)
Alternate Names
Alpha-L-iduronate sulfate sulfatase; EC 3.1.6.13; IDS; iduronate 2-sulfatase 14 kDa chain; iduronate 2-sulfatase 42 kDa chain; iduronate 2-sulfatase; idursulfase; MPS2; S; SIDS

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Citations for Human Iduronate 2-Sulfatase/IDS Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Molecular architecture determines brain delivery of a transferrin receptor–targeted lysosomal enzyme
    Authors: Annie Arguello, Cathal S. Mahon, Meredith E.K. Calvert, Darren Chan, Jason C. Dugas, Michelle E. Pizzo et al.
    Journal of Experimental Medicine
  2. iPS-derived neural stem cells for disease modeling and evaluation of therapeutics for mucopolysaccharidosis type II
    Authors: Junjie Hong, Yu-Shan Cheng, Shu Yang, Manju Swaroop, Miao Xu, Jeanette Beers et al.
    Experimental Cell Research
  3. Iduronate-2-sulfatase transport vehicle rescues behavioral and skeletal phenotypes in a mouse model of Hunter syndrome
    Authors: A Arguello, R Meisner, ER Thomsen, HN Nguyen, R Ravi, J Simms, I Lo, J Speckart, J Holtzman, TM Gill, D Chan, Y Cheng, CL Chiu, JC Dugas, M Fang, IA Lopez, H Solanoy, B Tsogtbaata, Y Zhu, A Bhalla, KR Henne, AG Henry, A Delucchi, S Costanzo, JM Harris, D Diaz, K Scearce-Le, PE Sanchez
    JCI Insight, 2021-10-08;6(19):.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Capture
  4. Dose-Dependent Prevention of Metabolic and Neurologic Disease in Murine MPS II by ZFN-Mediated In�Vivo Genome Editing
    Authors: K Laoharawee, RC DeKelver, KM Podetz-Ped, M Rohde, S Sproul, HO Nguyen, T Nguyen, SJ St Martin, L Ou, S Tom, R Radeke, KE Meyer, MC Holmes, CB Whitley, T Wechsler, RS McIvor
    Mol. Ther., 2018-03-10;26(4):1127-1136.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Identification of a small molecule that facilitates the differentiation of human iPSCs/ESCs and mouse embryonic pancreatic explants into pancreatic endocrine cells
    Authors: Y Kondo, T Toyoda, R Ito, M Funato, Y Hosokawa, S Matsui, T Sudo, M Nakamura, C Okada, X Zhuang, A Watanabe, A Ohta, N Inagaki, K Osafune
    Diabetologia, 2017-05-22;0(0):.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: ICC
  6. Localized donor cells in brain of a Hunter disease patient after cord blood stem cell transplantation.
    Authors: Araya K, Sakai N, Mohri I
    Mol. Genet. Metab., 2009-05-24;98(3):255-63.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot

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