Human ICAM-3/CD50 Biotinylated Antibody

Catalog #: BAF715 Datasheet / COA / SDS

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BAF715

All ICAM-3/CD50 Antibodies

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Human ICAM-3/CD50 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ICAM‑3/CD50 in Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) ICAM-1 is observed and no cross-reactivity with rhICAM-2, rhVCAM-1, and recombinant mouse VCAM-1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ICAM‑3/CD50
Extracellular domain

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human ICAM‑3/CD50 Fc Chimera (Catalog # 715-IC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-3/CD50

Intercellular Adhesion Molecule‑3 (ICAM‑3, CD50), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA‑1 (CD11a/CD18) and alpha _d/ beta ₂. ICAM‑3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM‑3 has not been identified in the mouse.

References