Human ICAM-3/CD50 Antibody

Catalog #: BBA28 Datasheet / COA / SDS

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BBA28

All ICAM-3/CD50 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ICAM-3/CD50 Antibody Summary

Specificity

Detects human ICAM‑3/CD50 in Western blots. ICAM‑3/CD50 was found not to recognize CHO cells expressing the human ICAM-1 protein. ICAM‑3/CD50 was found to recognize CHO cells expressing the human ICAM-3 protein. The epitope for ICAM‑3/CD50 is located on domain 4 of the human ICAM-3 molecule.

Source

Monoclonal Mouse IgG₁ Clone # Cal 3.34

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Recombinant human ICAM‑3/CD50 Fc Chimera

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human ICAM‑3/CD50 Fc Chimera (Catalog # 715-IC)

Flow Cytometry

0.25 µg/10⁶ cells

Human peripheral blood monocytes

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-3/CD50

Intercellular Adhesion Molecule-3 (ICAM-3, CD50), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alpha _d/ beta ₂.
ICAM‑3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in the mouse.

References