Human Heparan Sulfate 6‑O‑Sulfotransferase 2/HS6ST2 Antibody

Catalog #: AF2710
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2710
AF2710-SP

All Heparan Sulfate 6-O-Sulfotransferase 2/HS6ST2 Antibodies

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Heparan Sulfate 6‑O‑Sulfotransferase 2/HS6ST2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Heparan Sulfate 6-O-Sulfotransferase 2/HS6ST2 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Heparan Sulfate 6-O-Sulfotransferase 2/HS6ST2
Gln179-Arg605
Accession # Q96MM7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Heparan Sulfate6-O-Sulfotransferase 2/HS6ST2

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human HS6ST2, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Heparan Sulfate 6-O-Sulfotransferase 2/HS6ST2

HS6ST2 is a key enzyme in HS biosynthesis. There are at least three alternatively spliced forms, which include a long form (645 aa, NP_001070656), an intermediate form (605 aa, Q96MM7) with 40 aa deleted between Arg 316 and Asn 357 of the long form, and a short form starting at Met 147 of the intermediate form. Both the long and intermediate forms predict a type II membrane protein, consistent with its Golgi location. The long form of human HS6ST2 shares 94% and 86% aa sequence identities with that of chimpanzee, dog, mouse, rat, and chicken, respectively.

Entrez Gene IDs

90161 (Human)

Alternate Names

EC-1; heparan sulfate 6-O-sulfotransferase 2; HS6ST2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Heparan Sulfate 6‑O‑Sulfotransferase 2/HS6ST2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Multiplex genome editing of mammalian cells for producing recombinant heparin
Authors: BE Thacker, KJ Thorne, C Cartwright, J Park, K Glass, A Chea, BP Kellman, NE Lewis, Z Wang, A Di Nardo, ST Sharfstein, W Jeske, J Walenga, J Hogwood, E Gray, B Mulloy, JD Esko, CA Glass
Metabolic engineering, 2022-01-14;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot