Human Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody

Catalog #: MAB5057 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5057
MAB5057-SP

All Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 584308

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1
Pro38-Trp411
Accession # O60243

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human liver and human liver cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1

Heparan Sulfate is a highly sulfated polysaccharide that can be found on cell surface and extracellular matrix. It is usually covalently attached to a protein core as the glycan component of a proteoglycan. Heparan Sulfate interacts with a variety of proteins, such as growth factors, protease inhibitors, cytokines, lipoprotein lipase and viral envelope proteins, thus plays roles from cell growth, cell differentiation, cell motility, blood coagulation, lipid metabolism to viral infection (1, 2). Heparan Sulfate consists of repeating residues of uronic acid and N‑acetylglucosamine. The uronic acid residues can be sulfated at 2-O position by Heparan Sulfate 2-O Sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be sulfated at N-, 3-O, and 6-O positions by N‑deacetylase/N‑sulfotransferases (NDSTs), Heparan Sulfate 3-O and 6-O sulfotransferases (HS3STs and HS6STs) respectively. However, the reactions catalyzed by these sulfotransferases are normally incomplete on the whole chain of Heparan Sulfate. As a result, Heparan Sulfate displays enormous sequence diversity that allows it to interact with a wide spectrum of proteins differently. Among three HS6STs, HS6ST1 is the first to be cloned (3). Mice deficient of the HS6ST1 homologue gene showed embryonic lethality (4).

References

Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
Esko, J.D. and S.B. Selleck (2002) Annu. Rev. Biochem. 71:435.
Habuchi, H et al. (1998) J. Biol. Chem. 273:9208.
Habuchi, H et al. (2007) J. Biol. Chem. 282:15578.
Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
MacRae, I.J. et al. (2000) Biochemistry 39:1613.
Wu, Z.L. et al. (2002) Faseb J. 16:539.

Entrez Gene IDs

9394 (Human); 50785 (Mouse); 316325 (Rat)

Alternate Names

DKFZp547H098; EC 2.8.2; EC 2.8.2.-; FLJ25392; heparan sulfate 6-O-sulfotransferase 1; heparan-sulfate 6-sulfotransferase; HS6ST1; HS6ST-1; HS6STheparan-sulfate 6-O-sulfotransferase 1; MGC116899; MGC116901