Human HAI‑2 Ectodomain Antibody

Name: Human HAI‑2 Ectodomain Antibody
Brand: R&D Systems
SKU: MAB1106
Rating: 3.7 (3 reviews)

Catalog #: MAB1106
2 Citations 3 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1106
MAB1106-SP

All HAI-2 Antibodies

Product Details

Citations (2)

FAQs

Supplemental Products

Reviews (3)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HAI‑2 Ectodomain Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human (rh)HAI-2 in direct ELISAs and Western blots. In these formats, does not cross-react with rhHAI-1 or rmHAI-2B.

Source

Monoclonal Mouse IgG₁ Clone # 170018

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human HAI-2
aa 28-197
Accession # O43291

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human HAI‑2 (Catalog # 1106-PI)

Human HAI-2 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human HAI‑2 Biotinylated Antibody (Catalog # BAF1106)

Standard: Recombinant Human HAI-2A Protein, CF (Catalog # 1106-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAI-2

HGF Activator Inhibitor type II (HAI-2), also known as placental bikunin, is a Kunitz-type protease inhibitor. It is a type I membrane protein containing 2 Kunitz inhibitor domains in its extracellular region. In mouse, at least three isoforms of HAI-2 exist as a result of alternative splicing. Isoforms 2 and 3, also known as HAI-2B and HAI-2C, respectively, are both missing the first amino-terminal Kunitz inhibitor domain.

Long Name

HGF Activator Inhibitor Type 2

Entrez Gene IDs

10653 (Human); 20733 (Mouse)

Alternate Names

HAI2; HAI-2; HAI-2DIAR3; HAI2FLJ45571; Hepatocyte growth factor activator inhibitor type 2; Kop; kunitz-type protease inhibitor 2; PB; Placental bikunin; serine peptidase inhibitor, Kunitz type, 2; serine protease inhibitor, Kunitz type, 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Serine Proteases and Regulators

Citations for Human HAI‑2 Ectodomain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Epigenetic Silencing of SPINT2 Promotes Cancer Cell Motility via HGF-MET Pathway Activation in Melanoma.
Authors: Hwang S, Kim H, Min M, Raghunathan R, Panova I, Munshi R, Ryu B
J Invest Dermatol, 2015-04-24;135(9):2283-91.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
An epigenetic genome-wide screen identifies SPINT2 as a novel tumor suppressor gene in pediatric medulloblastoma.
Authors: Kongkham PN, Northcott PA, Ra YS
Cancer Res., 2008-12-01;68(23):9945-53.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P