Human HAI-2 Biotinylated Antibody

Catalog #: BAF1106 Datasheet / COA / SDS

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BAF1106

All HAI-2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HAI-2 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human HAI-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse HAI-2B and recombinant human HAI-1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human HAI-2A (R&D Systems, Catalog # 1106-PI)
Ala28-Lys197
Accession # O43291

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human HAI‑2

Human HAI-2 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human HAI‑2 Ectodomain Antibody (Catalog # MAB1106)

Standard: Recombinant Human HAI-2A Protein, CF (Catalog # 1106-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAI-2

Two alternatively spliced forms of HAI-2 have been found in human tissues (1). HAI-2A, the full-length molecule and also known as placental bikunin, is a major form expressed in human tissues. Encoded by the SPINT2 gene, HAI-2A consists of two Kunitz domains, and a C-terminal transmembrane domain (1 - 5). Both Kunitz domains can function as inhibitors independent of each other. In addition to HGF activator and trysin, HAI-2A strongly inhibits plasmin, tissue and plasma kallikreins, and factor XIa. In comparison, HAI-2A is a weaker inhibitor of factor VIIa-tissue factor, factors IXa, Xa, and XIIa.

References

Kataoka, H. et al. (2002) Biochem. Biophys. Res. Comm. 290:1096.
Kawaguchi, T. et al. (1997) J. Biol. Chem. 272:27558.
Marlor. C.W. et al. (1997) J. Biol. Chem. 272:12202.
Muller-Pillasch, F. et al. (1998) Biochim. Biophys. Acta 1395:88.
Delaria, K.A. et al. (1997) J. Biol. Chem. 272:12209.

Long Name

HGF Activator Inhibitor Type 2

Entrez Gene IDs

10653 (Human); 20733 (Mouse)

Alternate Names

HAI2; HAI-2; HAI-2DIAR3; HAI2FLJ45571; Hepatocyte growth factor activator inhibitor type 2; Kop; kunitz-type protease inhibitor 2; PB; Placental bikunin; serine peptidase inhibitor, Kunitz type, 2; serine protease inhibitor, Kunitz type, 2