Human GPR37L1 Antibody

Catalog #: MAB4449 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4449
MAB4449-SP

Detection of GPR37L1 in A172 Human Cell Line by Flow Cytometry.

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All GPR37L1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GPR37L1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GPR37L1. Stains human GPR37L1 transfectants but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2A Clone # 444532

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human GPR37L1
Accession # O60883

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of GPR37L1 antibody in A172 Human Cell Line antibody by Flow Cytometry.

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Detection of GPR37L1 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human GPR37L1 Monoclonal Antibody (Catalog # MAB4449, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR37L1

GPR37L1 (G-protein coupled receptor 37-like 1), also called ETBR-LP-2 (endothelin B receptor-like protein 2), is a 481 aa, orphan 7-transmembrane receptor with calculated molecular weight of 53 kDa. GPR37L1 is expressed in cerebellar Bergmann glia, cerebral cortex and internal capsule fibers in the central nervous system. It shows 39% aa identity with GPR37, a neuronal protein that accumulates in juvenile Parkinson’s disease neurons under endoplasmic reticulum stress. GPR37L1 does not bind known endothelin B family members. The extracellular portions of human and mouse GPR37L1 share 85% aa identity.

Long Name

G Protein-coupled Receptor 37-like 1

Entrez Gene IDs

9283 (Human); 171469 (Mouse); 252939 (Rat)

Alternate Names

endothelin B receptor-like protein 2; ET(B)R-LP-2; ETBRLP2; ETBR-LP-2; ETBR-LP-2endothelin type b receptor-like protein 2; G protein-coupled receptor 37 like 1; GPR37L1; G-protein coupled receptor 37 like 1; G-protein coupled receptor 37-like 1