Human GPR26 Antibody

Catalog #: MAB8449 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8449
MAB8449-SP

Detection of GPR26 in HEK293 Human Cell Line Transfected with Human GPR26 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GPR26 Antibody Summary

Species Reactivity

Human

Specificity

Stains human GPR26 transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG_2B Clone # 891318

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human GPR26
Met1-Glu337
Accession # Q8NDV2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of GPR26 in HEK293 Human Cell Line Transfected with Human GPR26 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GPR26 and eGFP was stained with either (A) Mouse Anti-Human GPR26 Monoclonal Antibody (Catalog # MAB8449) or (B) Mouse IgG_2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR26

GPR26 is a brain-specific orphan G Protein-Coupled Receptor (GPCR) of the rhodopsin family. It is frequently reduced or absent in glioma cells and biopsies. Glioblastomas are highly malignant brain tumors. GPR26 has been proposed to be a genetic indicator of primary glioblastoma, suggesting that it could be a suppressor of primary glioblastoma development.

Long Name

G Protein-coupled Receptor 26

Entrez Gene IDs

2849 (Human); 233919 (Mouse)

Alternate Names

DKFZp761H2121; FLJ33774; G protein-coupled receptor 26; GPR26; G-protein coupled receptor 26; MGC138216