Human GPR111 Antibody

Catalog #: MAB64941 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB64941-100
MAB64941-SP

Detection of Human GPR111 by Western Blot.

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Human GPR111 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GPR111 in direct ELISAs. Stains human GPR111 HEK293-transfected cells and does not stain irrelevant non-GPR111 transfectants by Flow Cytometry.

Source

Monoclonal Mouse IgG_2B Clone # 594522

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human GPR111
Cys19-Lys375
Accession # Q8IZF7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human GPR111 by Western Blot. Western blot shows lysates of CHO Chinese hamster ovary cell line mock transfected or tranfected with human GPR111. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GPR111 Monoclonal Antibody (Catalog # MAB64941) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GPR111 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of GPR111 in HEK293 Human Cell Line Transfected with Human GPR111 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) GPR111 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human GPR111 Monoclonal Antibody (Catalog # MAB64941) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG2B Isotype Control Antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR111

GPR111 (G-protein coupled receptor 111; also PRG20) is a 71 kDa (predicted), seven transmembrane (TM) member of the GPR-2 family, LN-7TM subfamily of molecules. It is reportedly expressed in lung, mammary gland and diencephalon. Human GPR111 is 642 amino acids (aa) in length. It contains an extended N-terminal extracellular region with a mucin like stalk (aa 1-383), followed by a series of seven TM domains and a short C-terminal cytoplasmic tail. The N-terminus possesses a GPS (GPCR proteolytic site) (aa 324-368) that likely generates a soluble cleavage product. GPR111 is considered an adhesion-type GPCR, and as such, is expected to form dimers, if not oligomers. There is one potential splice variant for GPR111. It shows a 92 aa substitution for aa 1-24 coupled to a 19 aa substitution for aa 622-642. Over aa 19-375, human GPR111 shares 68% aa identity with mouse GPR111.

Long Name

G-Protein-Coupled Receptor 111

Entrez Gene IDs

222611 (Human); 435529 (Mouse); 301269 (Rat)

Alternate Names

G protein-coupled receptor 111; GPR111; PGR20