Human GPR101 Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB7495R Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB7495R-025

Detection of GPR101 in HEK293 Human Cell Line Transfected with Human GPR101 and eGFP by Flow Cytometry.

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Human GPR101 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human GPR101 in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 909603

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human GPR101
Met1-Pro508
Accession # NP_473362

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

5 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of GPR101 in HEK293 Human Cell Line Transfected with Human GPR101 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GPR101 and eGFP was stained with either (A) Mouse Anti-Human GPR101 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB7495R) or (B) Mouse IgG₁Alexa Fluor 647 Isotype Control (Catalog # IC002R). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: GPR101

GPR101 (G-protein coupled receptor 101), is a member of a family of proteins that contain seven transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPR101 is an orphan G protein-coupled receptor of unknown function. mRNA for this gene is detected predominantly in the brain for both human and mouse. The expression of GPR101 is altered by pregnancy and lactation in the rat.

Long Name

G Protein-coupled Receptor 101

Entrez Gene IDs

83550 (Human); 245424 (Mouse); 317608 (Rat)

Alternate Names

G protein-coupled receptor 101; GPCR6; GPR101; probable G-protein coupled receptor 101

Product Datasheets

Product Datasheet

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.