Human Glut3 Biotinylated Antibody

Catalog #: BAM1415 Datasheet / COA / SDS

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BAM1415

All Glut3 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Glut3 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Glut3. Stains human Glut3-transfected NS0 and CHO cells, but not control transfectants. In flow cytometry, no cross‑reactivity with human Glut1 or Glut2 transfectants is observed.

Source

Monoclonal Mouse IgG_2B Clone # 202017

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Glut3
Met1-Val496
Accession # P11169

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

Human Glut3 transfected NS0 cells

Immunocytochemistry

8-25 µg/mL

Immersion fixed Caco-2 human colorectal adenocarcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glut3

Glut3 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut3 is the glucose transporter responsible for maintaining an adequate glucose supply to neurons (1, 2). It is also expressed in placenta and articular chondrocytes (3, 4).

References

Vannuci, S.J. et al. (1997) Glia 21:2.
Mueckler, M. et al. (1997) Biochem. Soc. Trans. 25:951.
Illsley, N.P. (2000) Placenta 21:14.
Mobasheri, A. (2002) Cell Biol. Int. 26:297.

Long Name

Glucose Transporter Type 3

Entrez Gene IDs

6515 (Human); 20527 (Mouse)

Alternate Names

FLJ90380; Glut3; GLUT-3; GLUT3Glucose transporter type 3, brain; SLC2A3; solute carrier family 2 (facilitated glucose transporter), member 3; solute carrier family 2, facilitated glucose transporter member 3