Human GATA-4 Biotinylated Antibody

Catalog #: BAF2606
4 Citations Datasheet / COA / SDS

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BAF2606

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GATA-4 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human GATA‑4 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human GATA-1, -2, -3, -5, and -6 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human GATA-4
Met27-Phe211
Accession # P43694

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human GATA‑4

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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GATA‑4 in iBJ6 iPS Cell Line. GATA-4 was detected in immersion fixed iBJ6, an induced pluripotent stem cell line, differentiated to endoderm using Goat Anti-Human GATA-4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2606) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATA-4

GATA-4 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-4 interacts with other transcription factors such as NKX2.5, and myocyte enhancer factor 2 to regulate cardiac myocyte-specific gene expression. Human GATA-4 share 93%, 93% and 92% amino acid sequence identity with porcine, rat and mouse GATA-4, respectively.

Entrez Gene IDs

2626 (Human)

Alternate Names

GATA binding protein 4; GATA4; GATA-4; GATA-binding factor 4; GATA-binding protein 4; MGC126629; transcription factor GATA-4

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human GATA-4 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Generation of a human induced pluripotent stem cell line from a patient with GM3 synthase deficiency using self-replicating RNA vector
Authors: Tonin, R;Feo, F;Falliano, S;Giunti, L;Calamai, M;Procopio, E;Mari, F;Sciruicchio, V;Conti, V;Fanelli, I;Bambi, F;Guerrini, R;Morrone, A;
Stem cell research
Species: Human
Sample Types: Whole Cells
Applications: Immunocytochemistry
Development of Human Adrenocortical Adenoma (HAA1) Cell Line from Zona Reticularis
Authors: HK Ghayee, Y Xu, H Hatch, R Brockway, AS Multani, T Gu, WB Bollag, A Turcu, WE Rainey, J Rege, K Nanba, VJ Bhagwandin, F Nwariaku, V Stastny, AF Gazdar, JW Shay, RJ Auchus, SG Tevosian
International Journal of Molecular Sciences, 2022-12-29;24(1):.
Species: Human
Sample Types: Whole Cell
Applications: ICC
Lumen Expansion Facilitates Epiblast-Primitive Endoderm Fate Specification during Mouse Blastocyst Formation
Authors: Allyson Quinn Ryan, Chii Jou Chan, François Graner, Takashi Hiiragi
Developmental Cell
Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells.
Authors: Drukker M, Tang C, Ardehali R
Nat. Biotechnol., 2012-05-27;30(6):531-42.
Species: Human
Sample Types: Whole Cells
Applications: ICC