Human GATA-3 Biotinylated Antibody

Catalog #: BAF2605
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF2605

1 Image

All GATA-3 Antibodies

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GATA-3 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human GATA-3 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human GATA-3
Pro135-Ser258
Accession # P23771

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human GATA‑3

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

View Larger

GATA‑3 in MCF-7 Human Cell Line. GATA-3 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Goat Anti-Human GATA-3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2605) for 3 hours at room temperature. Cells were stained with the Northern-Lights™ 557-conjugated Streptavidin (red, upper panel; Catalog # NL999) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATA-3

GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development and the maintenance of the differentiated state in epithelial cells.

Entrez Gene IDs

2625 (Human); 14462 (Mouse)

Alternate Names

131320; GATA binding protein 3; GATA3; GATA-3; GATA-binding factor 3; GATA-binding protein 3; HDRMGC5199; MGC2346; MGC5445; trans-acting T-cell-specific transcription factor GATA-3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human GATA-3 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Regional immunity in melanoma: immunosuppressive changes precede nodal metastasis.
Authors: Mansfield AS, Holtan SG, Grotz TE
Mod. Pathol., 2010-12-10;24(4):487-94.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P