Human FPRL2/FPR3 PE-conjugated Antibody

Catalog #: FAB3896P Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB3896P-100

Detection of FPRL2/FPR3 in HEK293 Human Cell Line Transfected with Human FPRL2/FPR3 and eGFP by Flow Cytometry.

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All FPRL2/FPR3 Antibodies

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Human FPRL2/FPR3 PE-conjugated Antibody Summary

Specificity

Detects human FPRL2. Stains human FPRL2 transfectants but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2B Clone # 374822

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human FPRL2
Met1-Met352
Accession # P25089

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of FPRL2/FPR3 in HEK293 Human Cell Line Transfected with Human FPRL2/FPR3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) FPRL2/FPR3 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human FPRL2/FPR3 PE-conjugated Monoclonal Antibody (Catalog # FAB3896P). Quadrant markers were set based Mouse IgG2B Isotype Control Antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: FPRL2/FPR3

FPRL2, also known as FMLP receptor-like 2, is a 40 kDa 7TM protein that is expressed on monocytes and dendritic cells. It interacts with formyl and acetyl peptides to attract these cells to sites of infection. FPRL2 signaling also inhibits DC maturation. Human FPRL2 shares 63% and 62% amino acid sequence identity with mouse and rat FPRL2, respectively.

Long Name

Formyl Peptide Receptor-like 2

Entrez Gene IDs

2359 (Human); 14294 (Mouse)

Alternate Names

FMLP-R-II; FMLPY; formyl peptide receptor 3; Formyl peptide receptor-like 2FMLP-related receptor II; FPR3; FPRH1; FPRH1FPRH2; FPRH2; FPRL2; FPRL2FML2_HUMAN; N-formyl peptide receptor 3; RMLP-R-I