Human FOLR3 Antibody

Name: Human FOLR3 Antibody
Brand: R&D Systems
SKU: MAB5319
Rating: 4 (2 reviews)

Catalog #: MAB5319 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5319
MAB5319-SP

Detection of Human FOLR3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FOLR3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FOLR3 in direct ELISAs and Western blots. In direct ELISAs, 10% cross-reactivity with recombinant human (rh) FOLR2 is observed and no cross-reactivity with rhFOLR4 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 553701

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human FOLR3 long isoform
Ala22-Ser245
Accession # NP_000795

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human FOLR3 by Western Blot. Western blot shows lysates of JEG‑3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human FOLR3 Monoclonal Antibody (Catalog # MAB5319) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for FOLR3 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FOLR3

Folate receptor 3 (FOLR3), also known as folate receptor gamma, is a 32 kDa protein that binds folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1). Mature FOLR3 is a glycosylated protein that contains an imperfect carboxy terminal GPI anchor sequence (2, 3). A fraction of FOLR3 is GPI-linked to the cell surface, but the majority is released as a secreted protein (2). Mature human FOLR3 shares 74% and 83% amino acid (aa) sequence identity with FOLR1 and FOLR2, respectively. Orthologs of human FOLR3 have not been described in mouse or rat. A genetic polymorphism generates a truncated isoform that lacks the carboxy terminal 139 aa (2, 4). FOLR3 is expressed by cells of the myelocyte and B lymphocyte lineage as well as by various carcinomas (2, 3). FOLR3 is more highly expressed in ovarian carcinomas than in breast carcinomas or mesotheliomas (5). Unlike other folate receptors, FOLR3 does not exhibit stereoselectivity in the binding of folates (3).

References