Human Flt-3/Flk-2 Antibody

Catalog #: MAB8123 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8123-100
MAB8123-SP

Flt-3/Flk-2 Binding to Flt-3 Ligand/FLT3L is Blocked by Flt-3/Flk-2 Antibody.

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All Flt-3/Flk-2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Flt-3/Flk-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Flt-3/Flk-2 in ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 1038615

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human embryonic kidney cell, HEK293-derived human Flt-3/Flk-2
Asn27-Asn541
Accession # P36888

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

In a functional ELISA binding assay, 0.075-0.75 ug/mL of this antibody will block the binding of recombinant human rhFlt-3 ligand (Catalog # 308-FK) to immobilized recombinant human Flt-3 (Catalog # 10284-F3) coated at 50 ng/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Flt-3/Flk-2 Binding to Flt-3 Ligand/FLT3L is Blocked by Flt-3/Flk-2 Antibody. In a functional ELISA binding assay, 0.075-0.75 ug/mL of this antibody will block 50% of the binding of 100 ng/mL of recombinant human rhFlt-3 ligand (308-FK) to immobilized recombinant human Flt-3 (10284-F3) coated at 50 ng/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Flt-3/Flk-2

The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a 130-155 kDa member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF (1). The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Human Flt-3 cDNA encodes a 993 amino acid (aa) type I membrane protein with a 26 aa signal peptide, a 515 aa extracellular domain (ECD) with 10 potential N-linked glycosylation sites, a 21 aa transmembrane domain and a 431 aa cytoplasmic domain. Within ECD human Flt-3 shares 85% and 82% aa sequence identity with mouse and rat Flt-3, respectively. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist (2).

References

Rosnet, O. et al. (1996) Acta. Haemato. 95:218.
Drexler, H.G. (1996) Leukemia 10:588.

Long Name

fms-like Tyrosine Kinase 3

Entrez Gene IDs

2322 (Human); 14255 (Mouse)

Alternate Names

CD135 antigen; CD135; EC 2.7.10; fetal liver kinase 2; FL cytokine receptor; FLK2; Flk-2; FLT3 receptor tyrosine kinase; Flt3; Flt-3; Fms-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; Stem cell tyrosine kinase 1; STK-1; STK1EC 2.7.10.1; Tyrosine-protein kinase receptor FLT3