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Human Ficolin-2 Biotinylated Antibody

Catalog #: BAF2428
3 Citations Datasheet / COA / SDS
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BAF2428
Product Details
Citations (3)
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Human Ficolin-2 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Ficolin-2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human Ficolin-3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Ficolin-2
Leu26-Ala313
Accession # Q15485
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Ficolin‑2 (Catalog # 2428-FC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ficolin-2

Human Ficolin-2 (fibrinogen/collagen-like; previously called L-ficolin or ficolin-B) is a member of the ficolin family of secreted pattern recognition proteins that participate in the lectin complement activation pathway (1‑4). Ficolin-2 is expressed in the liver and released into the circulation (2). The 35‑40 kDa, 313 amino acid (aa) human Ficolin-2 contains a 25 aa signal sequence, an N‑terminal collagen domain and a C‑terminal fibrinogen‑like domain that includes a calcium binding site and two potential N‑glycosylation sites. The collagen domain mediates trimer formation. Larger homo‑multimers are formed by disulfide links at the N‑terminus, the most prominent of which is a 12 subunit oligomer (3, 5). Ficolin‑2 binds microbial ligands that contain acetylated compounds (6). Notably, this includes N‑acetyl glucosamine in compounds such as lipoteichoic acid in gram-positive bacteria. It also binds fungal 1,3-beta -D-glucan (4, 7, 8). Pathogen recognition by Ficolin-2 initiates an immune response that involves calcium-dependent interaction of Ficolin-2 with the MBL-associated serine protease (MASP) complex. This complex cleaves C4 to activate the complement pathway (4, 7, 8). In a secondary role, Ficolin-2 is known to bind late apoptotic and necrotic cells, probably through the recognition of exposed DNA. This also activates the complement cascade that assists in clearance of cells (9, 10). Mature human Ficolin‑2 shares 70%, 72%, 76% and 78% aa identity with mouse, rat, cow and pig Ficolin-2, respectively. It shares 84% and 52% aa identity with human ficolin-1 and ficolin-3, respectively. Single nucleotide polymorphisms are common in human Ficolin‑2. Some affect serum concentration, while others can increase or decrease ligand binding (11).

References
  1. Endo, Y. et al. (2006) Adv. Exp. Med. Biol. 586:265.
  2. Endo, Y. et al. (1996) Genomics 36:515.
  3. Matsushita, M. et al. (1996) J. Biol. Chem. 271:2448.
  4. Ma, Y. G. et al. (2004) J. Biol. Chem. 279:25307.
  5. Hummelshoj, T. et al. (2007) Mol. Immunol. 44:401.
  6. Krarup, A. et al. (2004) J. Biol. Chem. 279:47513.
  7. Garlatti, V. et al. (2007) EMBO J. 26:623.
  8. Lynch, N. J. et al. (2004) J. Immunol. 172:1198.
  9. Jensen, M. L. et al. (2007) Mol. Immunol. 44:856.
  10. Kuraya, M. et al. (2005) Immunobiology 209:689.
  11. Hummelshoj, T. et al. (2005) Hum. Mol. Genet. 14:1651.
Long Name
Ficolin [Collagen/Fibrinogen Domain Containing] 2
Entrez Gene IDs
2220 (Human)
Alternate Names
37 kDa elastin-binding protein; Collagen/fibrinogen domain-containing protein 2; EBP-37ficolin (collagen/fibrinogen domain-containing lectin) 2 (hucolin); FCN2; FCNL; FCNLficolin B; ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin); Ficolin2; Ficolin-2; ficolin-B; ficolin-beta; Hucolin; L-Ficolin; P35; Serum lectin p35

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Citations for Human Ficolin-2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Rapid and efficient purification of ficolin-2 using a disposable CELLine bioreactor
    Authors: K. Aaron Geno, Brady L. Spencer, Moon H. Nahm
    Journal of Immunological Methods
  2. Blood Collection Tubes Influence Serum Ficolin-1 and Ficolin-2 Levels
    Authors: Allison M. Brady, Brady L. Spencer, Ann R. Falsey, Moon H. Nahm
    Clinical and Vaccine Immunology
  3. Synergy between ficolin-2 and pentraxin 3 boosts innate immune recognition and complement deposition.
    Authors: Ma YJ, Doni A, Hummelshoj T, Honore C, Bastone A, Mantovani A, Thielens NM, Garred P
    J. Biol. Chem., 2009-07-24;284(41):28263-75.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot

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