Human Fibronectin PE-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Fibronectin in HepG2 Human Cell Line by Flow Cytometry. HepG2 human liver cell line was stained with Mouse Anti-Human Fibronectin PE-conjugated Monoclonal Antibody (Catalog # IC1918P, filled histogram) or isotype control antibody (IC002, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.
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Detection of Fibronectin in WS‑1 Human Cell Line by Flow Cytometry. WS-1 human fetal skin fibroblast cell line was stained with Mouse Anti-Human Fibronectin PE-conjugated Monoclonal Antibody (Catalog # IC1918P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
Background: Fibronectin
Fibronectin is an extracellular matrix component that exists in different alternately spliced isoforms. Fibronectin mediates cell adhesion in its insoluble state but not as a soluble molecule. Fibronectins play a role in cell adhesion, migration, differentiation, and specific gene expression.
Product Datasheets
Citations for Human Fibronectin PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Dissecting the immunosuppressive tumor microenvironments in Glioblastoma-on-a-Chip for optimized PD-1 immunotherapy
Authors: Xin Cui, Chao Ma, Varshini Vasudevaraja, Jonathan Serrano, Jie Tong, Yansong Peng et al.
eLife
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A bioengineered immunocompetent human leukemia chip for preclinical screening of CAR T cell immunotherapy
Authors: Weiqiang Chen, Huishu Wang, Huishu Wang, Lunan Tong, Matthew T. Witkowski, Matthew Witkowski et al.
Research Square
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Fibronectin on the Surface of Myeloma Cell-derived Exosomes Mediates Exosome-Cell Interactions.
Authors: Purushothaman A, Bandari S, Liu J, Mobley J, Brown E, Sanderson R
J Biol Chem, 2015-11-24;291(4):1652-63.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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