Human Fibronectin Antibody Summary
Asn631-Pro705
Accession # P02751
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Fibronectin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB19182) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Fibronectin at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
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Fibronectin in HepG2 Human Cell Line. Fibronectin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line treated with monensin using Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB19182) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm (punctate). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Fibronectin in Human Liver Cancer Tissue. Fibronectin was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB19182) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in tumor cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fibronectin
Fibronectin is an extracellular matrix component that exists in different alternately spliced isoforms. Fibronectin mediates cell adhesion in its insoluble state but not as a soluble molecule. Fibronectins play a role in cell adhesion, migration, differentiation, and specific gene expression.
Product Datasheets
Citation for Human Fibronectin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Functionalized Graphene Nanoparticles Induce Human Mesenchymal Stem Cells to Express Distinct Extracellular Matrix Proteins Mediating Osteogenesis
Authors: SD Newby, T Masi, CD Griffin, WJ King, A Chipman, S Stephenson, DE Anderson, AS Biris, SE Bourdo, M Dhar
Int J Nanomedicine, 2020-04-15;15(0):2501-2513.
Species: Human
Sample Types: Whole Cells
Applications: ICC/IF
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