Human Fes Antibody

Catalog #: AF6460 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6460
AF6460-SP

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Human Fes Antibody Summary

Specificity

Detects human Fes in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Fes
aa 169-370
Accession # P07332

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Fes by Western Blot. Western blot shows lysates of HEL 92.1.7 human erythroleukemic cell line. PVDF Membrane was probed with 2 µg/mL of Goat Anti-Human Fes Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6460) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Fes at approximately 93 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fes

Fes (homolog of feline sarcoma oncogene), also called c-FES or c-FPS, is a 92 kDa protein within subgroup IV of the non-receptor tyrosine kinase family. It is expressed in myeloid hematopoietic cells, vascular endothelium and neurons and regulates cell adhesion and extravasation. The 822 amino acid (aa) human Fes contains F-BAR (aa 7-239), SH2 (aa 460-549) and kinase (aa 561-822) domains. The F-BAR domain contains coiled-coil homo-oligomerization and microtubule binding sequences. Within the region used as an immunogen, human Fes shares 85% and 87% aa identity with mouse and rat Fes, respectively. Human isoforms of 764, 752, 694 and 681 aa may be missing aa 72-129 or 441-511 and/or contain an alternate 17 aa for aa 610-640, while an isoform of 891 aa contains 18 and 51 aa inserted after aa 510 and 568.

Long Name

Feline Sarcoma Proto-oncogene

Entrez Gene IDs

2242 (Human); 14159 (Mouse); 361597 (Rat)

Alternate Names

EC 2.7.10; EC 2.7.10.2; feline sarcoma oncogene; feline sarcoma virus; Fes; FPS; Proto-oncogene c-Fes; Proto-oncogene c-Fps; proto-oncogene tyrosine-protein kinase Fes/Fps; tyrosine-protein kinase Fes/Fps