Human FCRL2/FcRH2, FCRL5/FcRH5 Antibody

Catalog #: MAB2048
2 Citations Datasheet / COA / SDS

Clone 296902 was used by HLDA to establish CD designation

Catalog #	Availability	Size / Price	Qty
MAB2048
MAB2048-SP

Detection of FCRL2/FcRH2, FCRL5/FcRH5 in Human PBMCs by Flow Cytometry.

1 Image

All FCRL2/FcRH2 Antibodies

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FCRL2/FcRH2, FCRL5/FcRH5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FCRL2/FcRH2 and FCRL5/FcRH5 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human FCRL1, FCRL3, and FCRL4 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 296902

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human FCRL2/FcRH2
Glu15-Asp395
Accession # Q96LA5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human FCRL2/FcRH2 (Catalog # 2048-FC)

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of FCRL2/FcRH2, FCRL5/FcRH5 in Human PBMCs by Flow Cytometry. Human peripheral blood monocytes (PBMC) were labeled with Mouse Anti-Human FCRL2/FcRH2, FCRL5/FcRH5 Monoclonal Antibody (Catalog # MAB2048) followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (F0101B) and Human CD19 PE-conjugated Monoclonal Antibody (FAB4867P). Quadrant markers were set based on control antibody staining (MAB002).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRL2/FcRH2

Fc receptor-like 2 (FCRL2), also known as FcRH2 and IRTA4, belongs to the family of glycoprotein homologs of classical immunoglobulin (Ig) Fc receptors. In human, the type I transmembrane FCRL protein family contains from three to nine immunoglobulin-like domains (1, 2). Mature human FcRH2 consists of a 382 amino acid (aa) extracellular domain (ECD) with four Ig-like C2-set domains, a 21 aa transmembrane segment, and an 86 aa cytoplasmic domain with one ITAM-like, and two ITIM-like motifs (3‑5). Alternate splicing of human FCRL2 may generate isoforms with N-terminal, internal, or C-terminal deletions (4, 5). The gene for FcRH2 maps to the human Iq21-23 locus which is a hotspot for chromosomal translocation events associated with B cell malignancies (3, 6). Although there are several Fc receptor-like genes in the mouse, none of these is a clear ortholog to human FCRL2 (7). FCRL proteins are differentially expressed among B cells (2). FCRL2 is preferentially expressed on naïve and CD27⁺ memory B cells within the spleen, lymph nodes, tonsils, and peripheral blood (3, 4, 8, 9). It is also expressed on most B cells in B cell chronic lymphocytic leukemia (B-CLL) patients (10). FCRL2 upregulation is associated with mutation of the immunoglobulin heavy chain variable (IGHV) and less aggressive forms of B-CLL (9, 11).

References

Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
Davis, R.S. et al. (2007) Annu. Rev. Immunol. 25:525.
Miller, I. et al. (2002) Blood 99:2662.
Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772.
Xu, M.J. et al. (2001) Biochem. Biophys. Res. Commun. 280:768.
Hatzivassiliou, G. et al. (2001) Immunity 14:277.
Davis, R.S. et al. (2004) Int. Immunol. 16:1343.
Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
Huttmann, A. et al. (2006) Leukemia 20:1774.
Kazemi, T. et al. (2008) Int. J. Cancer 123:2113.
Li, F.J. et al. (2008) Blood 112:179.

Long Name

Fc Receptor-like 2

Entrez Gene IDs

79368 (Human)

Alternate Names

CD307b antigen; CD307b; Fc receptor homolog 2; Fc receptor-like 2; FcRH2; FCRH2SPAP1B; FCRL2; FcR-like protein 2; IFGP family protein 4; IFGP4; Immunoglobulin receptor translocation-associated protein 4; immunoglobulin superfamily Fc receptor, gp42; IRTA4; IRTA4SPAP1C; SH2 domain containing phosphatase anchor protein 1; SH2 domain-containing phosphatase anchor protein 1Fc receptor-like protein 2; SPAP1; SPAP1A; SPAP1immune receptor translocation-associated protein 4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human FCRL2/FcRH2, FCRL5/FcRH5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

New B-cell CD molecules.
Authors: Matesanz-Isabel J, Sintes J, Llinas L, de Salort J, Lazaro A, Engel P
Immunol. Lett., 2010-10-07;134(2):104-12.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
Immunol. Lett., 2010-10-07;134(2):167-73.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry