Human Fc gamma RIII (CD16) Fluorescein-conjugated Antibody

Catalog #: FAB2546F Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB2546F-025
FAB2546F-100

Detection of Fc gamma RIII (CD16) in Human Blood Neutrophils by Flow Cytometry.

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Human Fc gamma RIII (CD16) Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Fc gamma RIIIA/B (CD16) in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Fc gamma RIIA and recombinant mouse Fc gamma RIII is observed.

Source

Monoclonal Mouse IgG_2A Clone # 245536

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIIA/B (CD16)
Thr20-Gln208
Accession # O75015

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Fc gamma RIII (CD16) in Human Blood Neutrophils by Flow Cytometry. Human peripheral blood neutrophils were stained with Mouse Anti-Human Fc gamma RIII (CD16) Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2546F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Fc gamma RIII (CD16)

Fc gamma RIIIA and B are low affinity receptors for polyvalent immune-complexed IgG. Fc gamma RIIIA is a transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages. Fc gamma RIIIB is a non-activating GPI-linked decoy receptor expressed on neutrophils and eosinophils.

Long Name

Fc gamma Receptor III

Entrez Gene IDs

14131 (Mouse); 102140945 (Cynomolgus Monkey)

Alternate Names

CD16; CD16A; Fc fragment of IgG receptor IIIa; Fc gamma RIII; FCG3; FCGR3; FcgRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20