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Human Erythropoietin/EPO Antibody

Catalog #: MAB287
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB287-SP
MAB287-100
MAB287-500
Cell Proliferation Induced by Erythropoietin/EPO and Neutralization by Human Erythropoietin/EPO Antibody.
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Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Erythropoietin/EPO Antibody Summary

Species Reactivity
Human
Specificity
Detects human Erythropoietin/EPO.
Source
Monoclonal Mouse IgG1 Clone # 9C21D11
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Erythropoietin/EPO
Accession # P01588
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize Erythropoietin/EPO-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.5-1.5 µg/mL in the presence of 0.3 units/mL Recombinant Human Erythropoietin/EPO (Tissue Culture Grade).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by Erythropoietin/EPO and Neutralization by Human Erythropoietin/EPO Antibody. View Larger

Cell Proliferation Induced by Erythropoietin/EPO and Neutralization by Human Erythropoietin/EPO Antibody. Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (Catalog # 287-TC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (0.3 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB287). The ND50 is typically 0.5-1.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Erythropoietin/EPO

Erythropoietin (EPO) a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells.

Entrez Gene IDs
2056 (Human); 13856 (Mouse); 24335 (Rat)
Alternate Names
ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2

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Citations for Human Erythropoietin/EPO Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Continuous Translation of Circularized mRNA Improves Recombinant Protein Titer
    Authors: A Costello, NT Lao, N Barron, M Clynes
    Metab. Eng., 2019-01-04;0(0):.
    Species: Transgenic Hamster
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  2. Effect of erythropoietin on primed leucocyte expression profile
    Authors: Mirko Pesce, Paolo Felaco, Sara Franceschelli, Lorenza Speranza, Alfredo Grilli, Maria Anna Anna De Lutiis et al.
    Open Biology
  3. Novel human renal proximal tubular cell line for the production of complex proteins.
    Authors: Fliedl L, Manhart G, Kast F, Katinger H, Kunert R, Grillari J, Wieser M, Grillari-Voglauer R
    J Biotechnol, 2014-02-16;176(0):29-39.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development (Capture)
  4. The role and regulation of erythropoietin (EPO) and its receptor in skeletal muscle: how much do we really know?
    Authors: Séverine Lamon, Aaron P. Russell
    Frontiers in Physiology
  5. Macrophages as novel target cells for erythropoietin
    Authors: Lilach Lifshitz, Galit Tabak, Max Gassmann, Moshe Mittelman, Drorit Neumann
    Haematologica
  6. Erythropoietin exerts anti-epileptic effects with the suppression of aberrant new cell formation in the dentate gyrus and upregulation of neuropeptide Y in seizure model of rats.
    Authors: Kondo A, Shingo T, Yasuhara T, Kuramoto S, Kameda M, Kikuchi Y, Matsui T, Miyoshi Y, Agari T, Borlongan CV, Date I
    Brain Res., 2009-08-18;1296(0):127-36.
    Species: Rat
    Sample Types: In Vivo
    Applications: Neutralization
  7. Erythropoietin inhibits respiratory epithelial cell apoptosis in a model of acute lung injury.
    Authors: MacRedmond R, Singhera GK, Dorscheid DR
    Eur. Respir. J., 2009-01-22;33(6):1403-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Functional and immunochemical characterisation of different antibodies against the erythropoietin receptor.
    Authors: Kirkeby A, van Beek J, Nielsen J, Leist M, Helboe L
    J Neurosci Methods, 2007-04-12;164(1):50-8.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  9. Multiplexed digital mRNA expression analysis profiles system-wide changes in mRNA abundance and responsiveness of UPR-specific gene expression changes during batch culture of recombinant Chinese Hamster Ovary cells
    Authors: R Maldonado-, AJ Dickson
    Biotechnol J, 2018-02-02;0(0):.
  10. Erythropoietin signaling increases neurogenesis and oligodendrogenesis of endogenous neural stem cells following spinal cord injury both in�vivo and in�vitro
    Authors: H Zhang, X Fang, D Huang, Q Luo, M Zheng, K Wang, L Cao, Z Yin
    Mol Med Rep, 2017-10-25;0(0):.

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