Human EN-RAGE/S100A12 Antibody

Catalog #: MAB10521 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10521
MAB10521-SP

Detection of Human EN‑RAGE/S100A12 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EN-RAGE/S100A12 Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human EN-RAGE in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 161205

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human EN-RAGE
Met1-Glu92
Accession # P80511

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Flow Cytometry

2.5 µg/10⁶ cells

Human whole blood

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human EN‑RAGE/S100A12 by Western Blot. Western blot shows lysate of human granulocytes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human EN-RAGE/S100A12 Monoclonal Antibody (Catalog # MAB10521) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for EN-RAGE/S100A12 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EN-RAGE/S100A12

EN-RAGE, also known as S100A12 and Calgranulin C, is a 10 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of EF-hand calcium-binding proteins. Like other S100 proteins, S100A12 is small and generally acidic (1‑3). EN-RAGE forms noncovalent homodimers in the absence of divalent cations (4‑6); calcium and zinc promote the formation of higher order assemblies including tetramers and hexamers (4, 7, 8). The ability of S100A12 to chelate zinc enables it to inhibit the zinc-dependent metalloproteases MMP-2, -3, and -9 (8). S100A12 also forms heterodimers with S100A9 and binds to RAGE (Receptor for Advanced Glycation End-products), Annexin V, and several cytosolic enzymes involved in energy metabolism (9, 10). The hexameric form of EN-RAGE in particular binds RAGE with high affinity (7). EN-RAGE induces a variety of inflammatory responses including the in vivo recruitment of neutrophils, monocytes, and mast cells and the activation of mast cells and vascular endothelial cells (9, 11‑14). EN-RAGE is found at elevated levels under inflammatory conditions such as asthma, gout, rheumatoid arthritis synovial fluid, and atherosclerosis (8, 12, 14). S100A12 also promotes neurite outgrowth in isolated hippocampal neurons (15). An ortholog of S100A12 has not been identified in rodents, but the human protein is functional in mice and rats (11‑16).

References

Santamaria-Kisiel, L. et al. (2006) Biochem. J. 396:201.
Leclerc, E. et al. (2009) Biochim. Biophys. Acta 1793:993.
Wicki, R. et al. (1996) Cell Calcium 20:459.
Moroz, O.V. et al. (2009) BMC Biochem. 10:11.
Miranda, L.P. et al. (2001) FEBS Lett. 488:85.
Vogl, T. et al. (1999) J. Biol. Chem. 274:25291.
Xie, J. et al. (2007) J. Biol. Chem. 282:4218.
Goyette, J. et al. (2009) J. Immunol. 183:593.
Hatakeyama, T. et al. (2004) Eur. J. Biochem. 271:3765.
Hofmann, M.A. et al. (1999) Cell 97:889.
Yang, Z. et al. (2001) J. Leukoc. Biol. 69:986.
Rouleau, P. et al. (2003) Clin. Immunol. 107:46.
Yan, W.X. et al. (2008) J. Biol. Chem. 283:13035.
Yang, Z. et al. (2007) J. Allergy Clin. Immunol. 119:106.
Mikkelsen, S.E. et al. (2001) J. Neurochem. 79:767.
Fuellen, G. et al. (2004) OMICS 8:334.

Long Name

Extracellular Newly Identified RAGE-binding Protein

Entrez Gene IDs

6283 (Human)

Alternate Names

CAAF1; CAAF1Neutrophil S100 protein; CAAFI; CAGC; CAGCS100 calcium binding protein A12 (calgranulin C); CAGCS100; Calcium-binding protein in amniotic fluid 1; Calgranulin C; calgranulin-C; CGRPEN-RAGE; ENRAGE; EN-RAGE; Extracellular newly identified RAGE-binding protein; MRP6; p6calgranulin C; protein S100-A12; S100 calcium binding protein A12; S100 calcium-binding protein A12 (calgranulin C); S100 calcium-binding protein A12; S100A12