Human EMR2 Antibody

Catalog #: AF4894
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4894
AF4894-SP

Cell Adhesion Mediated by EMR2 and Neutralization by Human EMR2 Antibody.

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All EMR2 Antibodies

Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EMR2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human EMR2 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows less than 1% cross-reactivity with recombinant mouse EMR1.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human EMR2
Gln24-Gln478 (Gln24 predicted)
Accession # NP_038475

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Neutralization

Measured by its ability to neutralize EMR2-mediated adhesion of the NIH‑3T3 mouse embryonic fibroblast cell line. Stacey, M. et al. (2003) Blood 102:29161. The Neutralization Dose (ND₅₀) is typically 1.5-6 µg/mL in the presence of 1.5 µg/mL Recombinant Human EMR2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Adhesion Mediated by EMR2 and Neutralization by Human EMR2 Antibody. Recombinant Human EMR2 (Catalog # 4894-EM), immobilized onto a microplate, supports the adhesion of the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human EMR2 (1.5 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human EMR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4894). The ND₅₀ is typically 1.5-6 µg/mL.

Western Blot

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Detection of Human EMR2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human EMR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4894) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EMR2 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EMR2

EMR2 (EGF-like module-containing mucin-like receptor 2; designated CD312) is a glycoprotein belonging to the EGF-TM7 family of adhesion-type class B 7‑transmembrane (TM) receptors. EGF-like sequences within long extracellular N-termini, and a GPS (G-protein proteolytic site) domain are characteristic of this family, which is mainly expressed on cells of the immune system (1, 2). The human EMR2 cDNA encodes an 823 amino acid (aa) protein with five EGF-like domains within the first 250 aa, followed by a mucin-like stalk, a GPS domain (aa 479-530) and a 7-TM sequence (aa 531-785). The GPS domain is the site of autocatalytic cleavage, forming two cleaved portions that remain non-covalently attached as a heterodimer (1, 3). Of the first 290 aa of human EMR2, 284 aa (97%) are identical with family member CD97, likely due to gene duplication (2). The portion of human EMR2 N-terminal to the GPS domain (aa 1-478) shares 64%, 59%, 48% and 45% aa identity with corresponding regions of canine EMR2, equine EMR2, mouse CD97 and rat CD97, respectively. Alternate splicing of EMR2 creates isoforms that contain 2-5 EGF-like domains. Only the 5-EGF form contains EGF4, which is necessary for calcium-dependent binding of the EMR2/CD97 ligand, chondroitin sulfate (CS) (2, 4‑6). None of the isoforms engage the CD97 ligand, CD55 (DAF). EMR2 is restricted to myeloid cells (1, 2). EMR2 expression increases as monocytes differentiate into macrophages, and decreases with differentiation into dendritic cells (5). Activation increases neutrophil EMR2 expression (5). EMR2 localizes to the leading edge of migrating neutrophils and plays an important role in migration, adhesion and superoxide production (7). It is also thought to facilitate specific interaction of myeloid cells with peripheral B lymphocytes which express CS (6).

References

Kwakkenbos, M.J. et al. (2004) Immunogenetics 55:655.
Lin, H.-H. et al. (2000) Genomics 67:188.
Lin, H.-H. et al. (2004) J. Biol. Chem. 279:31823.
Stacey, M. et al. (2003) Blood 102:2916.
Chang G.-W. et al. (2007) Biochem. Biophys. Res. Commun. 353:133.
Kwakkenbos, M.J. et al. (2005) J. Leukoc. Biol. 77:112.
Yona, S. et al. (2008) FASEB J. 22:741.

Long Name

EGF-like Module Containing, Mucin-like, Hormone Receptor-like 2

Entrez Gene IDs

30817 (Human)

Alternate Names

CD312; DKFZp781B135; egf-like module containing, mucin-like, hormone receptor-like 2; egf-like module containing, mucin-like, hormone receptor-like sequence 2; EGF-like module receptor 2; EGF-like module-containing mucin-like hormone receptor-like 2; EMR2

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COA

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Citations for Human EMR2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Serum FHR1 binding to necrotic-type cells activates monocytic inflammasome and marks necrotic sites in vasculopathies
Authors: S Irmscher, SR Brix, SLH Zipfel, LD Halder, S Mutlutürk, S Wulf, E Girdauskas, H Reichenspu, RAK Stahl, B Jungnickel, T Wiech, PF Zipfel, C Skerka
Nat Commun, 2019-07-04;10(1):2961.
Factor H-related protein 1 (FHR-1) is associated with atherosclerotic cardiovascular disease
Authors: S Irmscher, SLH Zipfel, LD Halder, L Ivanov, A Gonzalez-D, C Waldeyer, M Seiffert, FJ Brunner, M von der He, I Löschmann, S Wulf, D Czamara, N Papac-Mili, O Strau beta, S Lorkowski, H Reichenspu, MV Holers, NK Banda, T Zeller, EB Binder, CJ Binder, T Wiech, PF Zipfel, C Skerka
Scientific Reports, 2021-11-18;11(1):22511.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
G Protein-Coupling of Adhesion GPCRs ADGRE2/EMR2 and ADGRE5/CD97, and Activation of G Protein Signalling by an Anti-EMR2 Antibody
Authors: N Bhudia, S Desai, N King, N Ancellin, D Grillot, AA Barnes, SJ Dowell
Sci Rep, 2020-01-22;10(1):1004.
Species: Human
Sample Types: Cell Lysates, Whole Cell
Applications: Immunoprecipitation, Western Blot