Human EGR1 Antibody

Catalog #: MAB28181 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB28181-100
MAB28181-SP

2 Images

All EGR1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EGR1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human EGR1 in direct ELISAs.

Source

Monoclonal Rat IgG2a Clone # 406910

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

EGR1 in Human Hodgkin's Lymphoma. EGR1 was detected in immersion fixed paraffin-embedded sections of human Hodgkin's Lymphoma using Rat Anti-Human EGR1 Monoclonal Antibody (Catalog # MAB28181) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry

View Larger

EGR1 in Human Adenocarcinoma Tissue. EGR1 was detected in immersion fixed paraffin-embedded sections of human adenocarcinoma tissue using Rat Anti-Human EGR1 Monoclonal Antibody (Catalog # MAB28181) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: EGR1

Early Growth Response 1, (EGR1) belongs to the EGR family of zinc finger transcription factors and regulates the expression of several tumor suppressors. Its expression is induced by diverse signals that initiate growth and differentiation. EGR1 suppresses or activates cell growth depending on the tumor type. Within the region that includes its three zinc finger domains, human EGR1 shares 99% aa sequence identity with mouse and rat EGR1.

Long Name

Early Growth Response 1

Entrez Gene IDs

1958 (Human)

Alternate Names

AT225Transcription factor ETR103; early growth response 1; early growth response protein 1; EGR1; EGR-1; G0S30; KROX24; KROX-24; Nerve growth factor-induced protein A; NGFIA; NGFI-ATranscription factor Zif268; TIS8; ZIF-268; Zinc finger protein Krox-24; ZNF225; ZNF225Zinc finger protein 225